期刊论文详细信息
WATER RESEARCH 卷:175
Enantiomeric profiling of quinolones and quinolones resistance gene qnrS in European wastewaters
Article
Castrignano, Erika1,12  Yang, Zhugen1,13  Feil, Edward J.2  Bade, Richard3,4  Castiglioni, Sara8  Causanilles, Ana7,8  Gracia-Lor, Emma6,16  Hernandez, Felix4  Plosz, Benedek G.9,14  Ramin, Pedram9,10  Rousis, Nikolaos, I5  Ryu, Yeonsuk4  Thomas, Kevin, V5,15  de Voogt, Pim6,11  Zuccato, Ettore5  Kasprzyk-Hordern, Barbara1 
[1] Univ Bath, Fac Sci, Dept Chem, Bath BA2 7AY, Avon, England
[2] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[3] Univ Jaume 1, Res Inst Pesticides & Water, Avda Sos Baynat S-N, E-12071 Castellon de La Plana, Spain
[4] Univ South Australia, Sch Pharm & Med Sci, Adelaide, SA 5000, Australia
[5] Norwegian Inst Water Res NIVA, Gaustadalle 21, N-0349 Oslo, Norway
[6] Ist Ric Farmacol Mario Negri IRCCS, Dept Environm Hlth Sci, Via La Masa 19, I-20156 Milan, Italy
[7] KWR Watercycle Res Inst, Chem Water Qual & Hlth, POB 1072, NL-3430 BB Nieuwegein, Netherlands
[8] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, POB 94248, NL-1090 GE Amsterdam, Netherlands
[9] Tech Univ Denmark, Dept Environm Engn, Bldg 115, DK-2800 Lyngby, Denmark
[10] Tech Univ Denmark, Proc & Syst Engn Ctr PROSYS, Dept Chem & Biochem Engn, Bldg 229, DK-2800 Lyngby, Denmark
[11] Univ Amsterdam, IBED, Amsterdam, Netherlands
[12] Kings Coll London, Sch Populat Hlth & Environm Sci, Dept Analyt Environm & Forens Sci, London SE1 9NH, England
[13] Cranfield Univ, Sch Water Energy & Environm, Cranfield MK43 0AL, Beds, England
[14] Univ Bath, Dept Chem Engn, Bath BA2 7AY, Avon, England
[15] Univ Queensland, QAEHS, 20 Cornwall St, Woolloongabba, Qld 4102, Australia
[16] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, Ave Complutense S-N, Madrid, Spain
关键词: Chiral antibiotics;    Enantioselective analysis;    Wastewater-based epidemiology;    Antibiotic resistance;    (fluoro)quinolones;    Biomarkers;   
DOI  :  10.1016/j.watres.2020.115653
来源: Elsevier
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【 摘 要 】

Wastewater-based epidemiology (WBE) was applied for the first time in seven cities across Europe with the aim of estimating quinolones consumption via the analysis of human urinary metabolites in wastewater. This report is also the first pan-European study focussed on the enantiomeric profiling of chiral quinolones in wastewater. By considering loads of (fluoro)quinolones in wastewater within the context of human stereoselective metabolism, we identified cities in Southern Europe characterised by both high usage and direct disposal of unused ofloxacin. In Northern European cities, S-(-)-ofloxacin loads were predominant with respect to R-(+)-ofloxacin. Much more potent, enantiomerically pure S(-)-ofloxacin was detected in wastewaters from Southern European cities, reflecting consumption of the enantiomerically pure antibiotic. Nalidixic acid, norfloxacin and lomefloxacin were detected in wastewater even though they were not prescribed according to official prescription data. S,S-(-)-moxifloxacin and S,S-(-)-moxifloxacin-N-sulphate were detected in wastewater due to metabolism of moxifloxacin. For the first time, average population-normalised ulifloxacin loads of 22.3 and 1.5 mg day(-1) 1000 people(-1) were reported for Milan and Castellon as a result of prulifloxacin metabolism. Enrichment of flumequine with first-eluting enantiomer in all the samples indicated animal metabolism rather than its direct disposal. Fluoroquinolone loads were compared with qnrS gene encoding quinolone resistance to correlate usage of fluoroquinolone and prevalence of resistance. The highest daily loads of the qnrS gene in Milan corresponded with the highest total quinolone load in Milan proving the hypothesis that higher usage of quinolones is linked with higher prevalence of quinolone resistance genes. Utrecht, with the lowest quinolones usage (low daily loads) had also one of the lowest daily loads of the qnrS gene. However, a similar trend was not observed in Oslo nor Bristol where higher qnrS gene loads were observed despite low quinolone usage. (C) 2020 Elsevier Ltd. All rights reserved.

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