Optically triggered immune response through photocaged oligonucleotides

TETRAHEDRON LETTERS

卷:56

Article

Govan, Jeane M.¹  Young, Douglas D.¹  Lively, Mark O.²  Deiters, Alexander^1,3 

[1] N Carolina State Univ, Raleigh, NC 27167 USA

[2] Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC 27157 USA

[3] Univ Pittsburgh, Pittsburgh, PA 15260 USA

关键词: Oligonucleotide synthesis;    Light-activation;    Photocaged nucleotides;    Immune response;    Toll-like receptors;   

DOI  :  10.1016/j.tetlet.2015.01.165

来源: Elsevier

【 摘 要 】

Bacterial and viral CpG oligonculeotides are unmethylated cytosine-phosphate-guanosine dinucleotide sequences and trigger an innate immune response through activation of the toll-like receptor 9 (TLR9). We have developed synthetic photocaged CpGs via site-specific incorporation of nitropiperonyloxymethyl (NPOM)-caged thymidine residues. These oligonucleotides enable the optical control of TLR9 function and thereby provide light-activation of an immune response. We provide a proof-of-concept model by applying a reporter assay in live cells and by quantification of endogenous production of interleukin 6. (C) 2015 Elsevier Ltd. All rights reserved.

【 授权许可】

Free