Evaluation of traditional plant extracts for innate immune mechanisms and disease resistance against fish bacterial Aeromonas hydrophila and Pseudomonas sp.

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Hardi, E.H.^1 ; Saptiani, G.^1 ; Kusuma, I.W.^1 ; Suwinarti, W.^1 ; Nugroho, R.A.^1

Department of Aquaculture, Faculty of Agriculture, Mulawarman University, Jl. Gunung Tabur, Gunung Kelua, Samarinda Ulu, East Kalimantan Samarinda, Indonesia^1

关键词: Aeromonas hydrophila;    Disease resistance;    Herbal extract;    Immune response;    Injection method;    Innate immune response;    Innate immunes;    Oral administration;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012003/pdf
DOI  :  10.1088/1755-1315/139/1/012003

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

The purposes of this study were to evaluate effect of ethanol herbal extracts of Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet on Tilapia (Oreochromis nilaticus) innate immune mechanisms and disease resistance against Aeromonas hydrophila and Pseudomonas sp. Fish were intramuscularly injected with 0.1 mL/fish (1010CFU mL-1) of each bacterium on the day 6thof post treatment using extract by several methods (injection, oral administration and immersion). The doses of extract were 600 ppm of B. pandurata, 900 ppm S. ferox and 200 ppm of Z. zerumbet. The percentage mortality, Relative Percent Survival (RPS) and innate immune response were assessed on weeks 1, 2, 3 and 4. All the methods were effective to enhance the immune parameters after 2 weeks application and the RPS of treatment reached more than 90 %. The results showed that the injection method of extracts was the most effective method to control A. hydrophila and Pseudomonas sp. The result indicated that all the doses of extracts could be significantly influence the immune response and protect the health status of tilapia against A. hydrophila and Pseudomonas sp. infections.