Synthesis and biological activity of a fluorescent analogue of 2—5A

FEBS Letters

Lesiak, Krystyna¹  Torrence, Paul F.¹ 

[1] Laboratory of Chemistry, National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases, US National Institutes of Health, Bethesda, MD 20205, USA

关键词: Interferon;    2—5A;    2—5A-Activated endonuclease;    Ethenoadenosine;    Fluorescence;    Oligonucleotide synthesis;    ϵA;    1;    N 6-ethenoadenosine i.e.;    (3-β-D-ribofuranosylimidazo[2.1-i]purine);    2';    5'(pϵA)3;    p5′ϵA2′p5′ϵA2′p5′ϵA;    2′;    5′pp(pϵA)3;    ppp5′ϵA2′p5′ϵA2′p5′ϵA;    2—5A;    ppp5′A2′p5′(A2′p5′) n A;    where n⩾1-≈10;   

DOI  :  10.1016/0014-5793(83)80089-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fluorescent analogue of 2—5A, ppp5′ ϵA2′p5′ϵA2′p5′ϵA, was obtained by reaction of 2′, 5′ (pA)₃ with chloroacetaldehyde followed by conversion to the 5-triphosphate through reaction of the corresponding phosphoroimidazolide with pyrophosphate anion. The 5′-monophosphate, p5′ϵA2′p5′ϵA2′p5′ϵA, was not an antagonist of 2—5A action in extracts of mouse L cells. Neither did the 5′-triphosphate, ppp5′ϵA2′p5′ϵA2′p5′ϵA, inhibit translation. Moreover, the 5′-triphosphate was bound to the 2—5A-dependent endonuclease 5000-times less effectively than 2—5A itself.

【 授权许可】

Unknown