Targeting phosphoinositide signaling in cancer: relevant techniques to study lipids and novel avenues for therapeutic intervention

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Ryan M. Loughran¹  Alicia Llorente¹  Brooke M. Emerling² 

[1] Cancer Metabolism and Microenvironment Program, Sanford Burnham Prebys, La Jolla, CA, United States;null;

关键词: phosphoinositide;    lipid;    metabolism;    cancer;    signaling;    PI5P4K;    lipidomics;    mass spectometry;   

DOI  :  10.3389/fcell.2023.1297355

 received in 2023-09-19, accepted in 2023-10-12,  发布年份 2023

来源: Frontiers

【 摘 要 】

Phosphoinositides serve as essential players in numerous biological activities and are critical for overall cellular function. Due to their complex chemical structures, localization, and low abundance, current challenges in the phosphoinositide field include the accurate measurement and identification of specific variants, particularly those with acyl chains. Researchers are intensively developing innovative techniques and approaches to address these challenges and advance our understanding of the impact of phosphoinositide signaling on cellular biology. This article provides an overview of recent advances in the study of phosphoinositides, including mass spectrometry, lipid biosensors, and real-time activity assays using fluorometric sensors. These methodologies have proven instrumental for a comprehensive exploration of the cellular distribution and dynamics of phosphoinositides and have shed light on the growing significance of these lipids in human health and various pathological processes, including cancer. To illustrate the importance of phosphoinositide signaling in disease, this perspective also highlights the role of a family of lipid kinases named phosphatidylinositol 5-phosphate 4-kinases (PI5P4Ks), which have recently emerged as exciting therapeutic targets for cancer treatment. The ongoing exploration of phosphoinositide signaling not only deepens our understanding of cellular biology but also holds promise for novel interventions in cancer therapy.

【 授权许可】

Unknown   
Copyright © 2023 Llorente, Loughran and Emerling.