Novel Hydrogen Production Systems Operative at Thermodynamic Extremes

Gunsalus, Robert

关键词: Syntrophic bacteria;    hydrogen metabolism;    metabolism;    electron flow;   

DOI  :  10.2172/1057055
RP-ID  :  DOE/ER64689-8
PID  :  OSTI ID: 1057055

学科分类：再生能源与代替技术

美国|英语

来源: SciTech Connect

【 摘 要 】

We have employed a suite of molecular, bioinformatics, and biochemical tools to interrogate the thermodynamically limiting steps of H{sub 2} production from fatty acids in syntrophic communities. We also developed a new microbial model system that generates high H{sub 2} concentrations (over 17% of the gas phase) with high H{sub 2} yields of over 3 moles H{sub 2} per mole glucose. Lastly, a systems-based study of biohydrogen production in model anaerobic consortia was performed to begin identifying key regulated steps as a precursor to modeling co-metabolism. The results of these studies significantly expand our ability to predict and model systems for H{sub 2} production in novel anaerobes that are currently very poorly documented or understood.