Regional sex differences in neurochemical profiles of healthy mice measured by magnetic resonance spectroscopy at 9.4 tesla

Frontiers in Neuroscience

Neuroscience

Gülin Öz¹  Sarah Larson¹  Ivan Tkáč¹  Lynn E. Eberly²  Harry T. Orr³  Hayley S. McLoughlin⁴  Janet M. Dubinsky⁵  Rocio Gomez-Pastor⁵  Tiankai Xie⁶  Nitya Shah⁶ 

[1] Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN, United States;Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN, United States;Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN, United States;Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, United States;Department of Neurology, University of Michigan, Ann Arbor, MI, United States;Department of Neuroscience, University of Minnesota, Minneapolis, MN, United States;Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN, United States;

关键词: metabolite;    taurine;    glucose;    total creatine;    ascorbate;    glutamate;   

DOI  :  10.3389/fnins.2023.1278828

 received in 2023-08-16, accepted in 2023-10-03,  发布年份 2023

来源: Frontiers

【 摘 要 】

ObjectiveTo determine sex differences in the neurochemical concentrations measured by in vivo proton magnetic resonance spectroscopy (1H MRS) of healthy mice on a genetic background commonly used for neurodegenerative disease models.Methods1H MRS data collected from wild type mice with C57BL/6 or related genetic backgrounds in seven prior studies were used in this retrospective analysis. To be included, data had to be collected at 9.4 tesla magnetic field using advanced 1H MRS protocols, with isoflurane anesthesia and similar animal handling protocols, and a similar number of datasets from male and female mice had to be available for the brain regions analyzed. Overall, 155 spectra from female mice and 166 spectra from male mice (321 in total), collected from six brain regions (brainstem, cerebellum, cortex, hippocampus, hypothalamus, and striatum) at various ages were included.ResultsConcentrations of taurine, total creatine (creatine + phosphocreatine), ascorbate, glucose and glutamate were consistently higher in male vs. female mice in most brain regions. Striatum was an exception with similar total creatine in male and female mice. The sex difference pattern in the hypothalamus was notably different from other regions. Interaction between sex and age was significant for total creatine and taurine in the cerebellum and hippocampus.ConclusionSex differences in regional neurochemical levels are small but significant and age-dependent, with consistent male–female differences across most brain regions. The neuroendocrine region hypothalamus displays a different pattern of sex differences in neurochemical levels. Differences in energy metabolism and cellular density may underlie the differences, with higher metabolic rates in females and higher osmoregulatory and antioxidant capacity in males.

【 授权许可】

Unknown   
Copyright © 2023 Tkáč, Xie, Shah, Larson, Dubinsky, Gomez-Pastor, McLoughlin, Orr, Eberly and Öz.