Capparis cartilaginea decne (capparaceae): isolation of flavonoids by high-speed countercurrent chromatography and their anti-inflammatory evaluation

Frontiers in Pharmacology

Pharmacology

Bruna Govoni¹  Hugo Verli¹  Wirginia Kukula-Koch²  Fabio Boylan³  Bashaer Alsharif⁴  Nadhim Hante⁵  María Jose Santos-Martinez⁶ 

[1] Center of Biotechnology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil;Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, Lublin, Poland;School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland;School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland;Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia;School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland;Faculty of Pharmacy, University of Kufa, Al-Najaf, Iraq;School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Ireland;School of Medicine, Trinity College Dublin, Dublin, Ireland;

关键词: flavonoids;    capparis;    HSCCC;    anti-inflammatory;    LC-MS;    virtual screening;   

DOI  :  10.3389/fphar.2023.1285243

 received in 2023-08-29, accepted in 2023-10-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Capparis cartilaginea Decne. (CC) originates from the dry regions of Asia and the Mediterranean basin. In traditional medicine, tea of CC leaves is commonly used to treat inflammatory conditions such as rheumatism, arthritis, and gout. Due to the limited studies on the phytochemistry and biological activity of CC compared to other members of the Capparaceae family, this work aims to: 1) Identify the chemical composition of CC extract and 2) Investigate the potential anti-inflammatory effect of CC extract, tea and the isolated compounds.Methods: To guarantee aim 1, high-speed countercurrent chromatography (HSCC) method; Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography coupled to Electrospray Ionisation and Quadrupole Time-of-Flight Mass Spectrometry (HPLC-ESIQTOF-MS/MS) were employed for this purpose. To guarantee aim 2, we studied the effect of the isolated flavonoids on matrix metalloproteinases (MMPs) −9 and −2 in murine macrophages. Molecular docking was initially performed to assess the binding affinity of the isolated flavonoids to the active site of MMP-9.Results and discussion: In silico model was a powerful tool to predict the compounds that could strongly bind and inhibit MMPs. CC extract and tea have shown to possess a significant antioxidant and anti-inflammatory effect, which can partially explain their traditional medicinal use.

【 授权许可】

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Copyright © 2023 Alsharif, Hante, Govoni, Verli, Kukula-Koch, Jose Santos-Martinez and Boylan.