Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography

Química Nova

Daniel Rinaldo²  Marcelo Aparecido Silva²  Clenilson Martins Rodrigues²  Tamara Regina Calvo²  Miriam Sannomiya²  Lourdes Campaner Dos Santos²  Wagner Vilegas²  Hélio Kushima¹  Clélia Akiko Hiruma-lima¹  Alba Regina Monteiro De Souza Brito¹ 

[1] ,Universidade Estadual Paulista Instituto de Química Departamento de Química OrgânicaAraraquara SP ,Brasil

关键词: HSCCC;    Davilla elliptica;    flavonoids;   

DOI  :  10.1590/S0100-40422006000500011

来源: SciELO

【 摘 要 】

High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.

【 授权许可】

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