Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography

Química Nova

Universidade Estadual Paulista, Botucatu, Brasil¹  Rinaldo, Daniel¹  Universidade Estadual Paulista, Araraquara, Brasil¹  Vilegas, Wagner¹  Brito, Alba Regina Monteiro de Souza¹  Kushima, Hélio¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Calvo, Tamara Regina¹  Silva, Marcelo Aparecido¹  Sannomiya, Miriam¹  Hiruma-Lima, Clélia Akiko¹  Santos, Lourdes Campaner dos¹  Rodrigues, Clenilson Martins¹ 

关键词: HSCCC;    Davilla elliptica;    flavonoids.;   

DOI  :  10.1590/S0100-40422006000500011

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-a-L-rhamnopyranoside and myricetin-3-O-a-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by 1H NMR, 13C NMR and HPLC-UV-DAD analyses.

【 授权许可】

Unknown