Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography

Química Nova

Miriam Sannomiya¹  Marcelo Aparecido Silva¹  Tamara Regina Calvo¹  Clélia Akiko Hiruma-Lima¹  Lourdes Campaner dos Santos¹  Wagner Vilegas¹  Hélio Kushima¹  Daniel Rinaldo¹  Clenilson Martins Rodrigues¹  Alba Regina Monteiro de Souza Brito² 

[1] Universidade Estadual Paulista;Universidade Estadual de Campinas;

关键词: HSCCC;    Davilla elliptica;    flavonoids;   

DOI  :  10.1590/S0100-40422006000500011

来源: DOAJ

【 摘 要 】

High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.

【 授权许可】

Unknown