A unique dual acyltransferase system shared in the polyketide chain initiation of kidamycinone and rubiflavinone biosynthesis

Frontiers in Microbiology

Microbiology

Jun-Pil Jang¹  Jae-Hyuk Jang¹  Young-Soo Hong¹  Kyung Taek Heo¹  Gwi Ja Hwang¹  Beomcheol Park²  Byeongsan Lee²  Bang Yeon Hwang³ 

[1] Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju-si, Republic of Korea;Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju-si, Republic of Korea;College of Pharmacy, Chungbuk National University, Cheongju-si, Republic of Korea;College of Pharmacy, Chungbuk National University, Cheongju-si, Republic of Korea;

关键词: biosynthesis;    natural products;    polyketide synthase;    dual acyltransferase;    Streptomyces;   

DOI  :  10.3389/fmicb.2023.1274358

 received in 2023-08-08, accepted in 2023-10-04,  发布年份 2023

来源: Frontiers

【 摘 要 】

The pluramycin family of natural products has diverse substituents at the C2 position, which are closely related to their biological activity. Therefore, it is important to understand the biosynthesis of C2 substituents. In this study, we describe the biosynthesis of C2 moieties in Streptomyces sp. W2061, which produces kidamycin and rubiflavinone C-1, containing anthrapyran aglycones. Sequence analysis of the loading module (Kid13) of the PKS responsible for the synthesis of these anthrapyran aglycones is useful for confirming the incorporation of atypical primer units into the corresponding products. Kid13 is a ketosynthase-like decarboxylase (KSQ)-type loading module with unusual dual acyltransferase (AT) domains (AT1-1 and AT1-2). The AT1-2 domain primarily loads ethylmalonyl-CoA and malonyl-CoA for rubiflavinone and kidamycinone and rubiflavinone, respectively; however, the AT1-1 domain contributed to the functioning of the AT1-2 domain to efficiently load ethylmalonyl-CoA for rubiflavinone. We found that the dual AT system was involved in the production of kidamycinone, an aglycone of kidamycin, and rubiflavinone C-1 by other shared biosynthetic genes in Streptomyces sp. W2061. This study broadens our understanding of the incorporation of atypical primer units into polyketide products.

【 授权许可】

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Copyright © 2023 Heo, Lee, Hwang, Park, Jang, Hwang, Jang and Hong.