Brief research report: OM-85 reduces SARS-COV-2 viral RNA expression in nasopharyngeal cells from COVID-19 patients

Frontiers in Virology

Virology

João Ismael Budelon Gonçalvez¹  Gisele Cassão¹  Krist Helen Antunes¹  Ana Paula Duarte de Souza¹  Leonardo Duarte Santos¹  Bruno Lopes Abbadi²  Cristiano Valim Bizarro²  Pablo Machado²  Luiz Augusto Basso²  Christian Pasquali³  Renato T. Stein⁴ 

[1] Laboratory of Clinical and Experimental Immunology, Health and Life Science School – Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil;Molecular and Functional Biology Research Center (CPBMF), PUCRS, Porto Alegre, Brazil;Preclincal Research Department, OM-Pharma, Geneva, Switzerland;School of Medicine, PUCRS, Porto Alegre, Brazil;

关键词: COVID-19;    SARS-CoV-2;    OM-85;    nasopharyngeal;    RIG-I;    epithelial cells;   

DOI  :  10.3389/fviro.2023.1111619

 received in 2022-11-29, accepted in 2023-10-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety profile, widely used to prevent recurrent respiratory tract infections. Several studies have been reporting the immunomodulating properties and antiviral roles of OM-85. The COVID-19 pandemic, originating in 2019, has presented a significant global public health crisis. While effective vaccines have been developed, vaccination rates vary considerably, and numerous concerning viral variants continue to emerge. The challenge persists in creating early interventions to halt the progression of the disease to its severe stages. To examine the therapeutic effect of OM-85 after SARS-CoV-2 infection and compared to recombinant human (rhINF-β) we collected nasopharyngeal cells from COVID-19 patients. The cells were treated ex-vivo with OM-85 or hrINF-β and the response was analyzed after 24h for gene expression by real-time PCR. We found that OM-85 decreased the SARS-CoV-2 N1 gene expression and increased RIG-I (DDX58) in these cells. The expression of ACE2 was undetected in these samples. These data support the antiviral effect of OM-85 against SARS-CoV-2.

【 授权许可】

Unknown   
Copyright © 2023 Cassão, Antunes, Gonçalvez, Santos, Abbadi, Bizarro, Machado, Basso, Pasquali, Stein and de Souza