Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2

Viruses

Hervé Perrier¹  Paul Tramini²  Florence Carrouel³  Denis Bourgeois³  Claude Dussart³  Maude Bouscambert-Duchamp⁴  Martine Valette⁴ 

[1] Clinical Research Unit, Protestant Infirmary, 69300 Caluire-et-Cuire, France;Department of Public Health, Faculty of Dental Medicine, University of Montpellier, 34090 Montpellier, France;Laboratory “Health Systemic Process”, EA4129, University Lyon 1, 69008 Lyon, France;Virology Laboratory, Institute of Infectious Agents, Croix-Rousse Hospital, Hospices Civils of Lyon, 69004 Lyon, France;

关键词: saliva;    viral load;    COVID-19;    nasopharyngeal;    SARS-CoV-2;   

DOI  :  10.3390/v13050895

来源: DOAJ

【 摘 要 】

The aim of this study was to determine whether self-collected pure saliva (SCPS) is comparable to nasopharyngeal (NP) swabs in the quantitative detection of SARS-CoV-2 by RT-PCR in asymptomatic, mild patients with confirmed COVID-19. Thirty-one patients aged from 18 to 85 years were included between 9 June and 11 December 2020. A SCPS sample and a NP sample were taken for each patient. Quantitative PCR was performed to detect SARS-CoV-2 viral load. Results of SCPS vs. NP samples testing were compared. Statistical analyses were performed. Viral load was significantly correlated (r = 0.72). The concordance probability was estimated at 73.3%. In symptomatic adults, SCPS performance was similar to that of NP swabs (Percent Agreement = 74.1%; p = 0.11). Thus, the salivary test based on pure oral saliva samples easily obtained by noninvasive techniques has a fair agreement with the nasopharyngeal one in asymptomatic, mild patients with a confirmed diagnosis of COVID-19.

【 授权许可】

Unknown