Properties of three collagen scaffolds in comparison with native connective tissue: an in-vitro study

International Journal of Implant Dentistry

Research

Stefan P. Hicklin¹  Patrick R. Schmidlin¹  Kai R. Fischer¹  Alex Solderer¹  Nicole Widmer¹  Andrea Gubler¹ 

[1] Division for Periodontology and Peri-Implant Diseases, Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland;

关键词: Collagen matrix;    Acellular dermal matrix;    Connective tissue graft;    Resorption rate;    Scanning electron microscope (SEM);    Artificial saliva;    Simulated body fluid;   

DOI  :  10.1186/s40729-023-00504-z

 received in 2023-05-31, accepted in 2023-10-04,  发布年份 2023

来源: Springer

【 摘 要 】

PurposeTo evaluate collagen scaffolds (CS) in terms of their in vitro resorption behavior, surface structure, swelling behavior, and mechanical properties in physiologically simulated environments, compared with porcine native connective tissue.Materials and methodsThree test materials—one porcine collagen matrix (p-CM), two acellular dermal matrices (porcine = p-ADM, allogenic = a-ADM)—and porcine native connective tissue (p-CTG) as a control material were examined for resorption in four solutions using a high-precision scale. The solutions were artificial saliva (AS) and simulated body fluid (SBF), both with and without collagenase (0.5 U/ml at 37 °C). In addition, the surface structures of CS were analyzed using a scanning electron microscope (SEM) before and after exposure to AS or SBF. The swelling behavior of CS was evaluated by measuring volume change and liquid absorption capacity in phosphate-buffered saline (PBS). Finally, the mechanical properties of CS and p-CTG were investigated using cyclic compression testing in PBS.ResultsSolutions containing collagenase demonstrated high resorption rates with significant differences (p < 0.04) between the tested materials after 4 h, 8 h and 24 h, ranging from 54.1 to 100% after 24 h. SEM images revealed cross-linked collagen structures in all untreated specimens. Unlike a-ADM, the scaffolds of p-CM and p-ADM displayed a flake-like structure. The swelling ratio and fluid absorption capacity per area ranged from 13.4 to 25.5% among the test materials and showed following pattern: p-CM > a-ADM > p-ADM. P-CM exhibited higher elastic properties than p-ADM, whereas a-ADM, like p-CTG, were barely compressible and lost structural integrity under increasing pressure.Conclusions and clinical implicationsCollagen scaffolds vary significantly in their physical properties, such as resorption and swelling behavior and elastic properties, depending on their microstructure and composition. When clinically applied, these differences should be taken into consideration to achieve the desired outcomes.Graphical Abstract

【 授权许可】

CC BY   
© Deutsche Gesellschaft für Implantologie im Zahn‐, Mund‐ und Kieferbereich e.V., Japanese Society of Oral Implantology 2023

【 图 表 】

Fig. 3

12951_2017_255_Article_IEq34.gif

12936_2016_1316_Article_IEq8.gif

Fig. 1