Evaluation of oral keratinocyte progenitor and T-lymphocite cells response during early healing after augmentation of keratinized gingiva with a 3D collagen matrix - a pilot study

BMC Oral Health

Research Article

Octavia Vela¹  Maria Greabu²  Bogdan Calenic³  Horia Calniceanu⁴  Virgil Paunescu⁵  Florina Bojin⁵  Simona Anghel⁵  Marius Boariu⁶  Darian Rusu⁷  Stefan-Ioan Stratul⁷  Alexander Kralev⁷ 

[1] Dental Clinic Dr.Stratul, Str.Emanoil Gojdu, nr.5, 300176, Timisoara, Romania;Department of Biochemistry, Faculty of Dental Medicine, University of Medicine and Pharmacy, Carol Davila, Blvd-ul Eroii Sanitari, No 8, Bucharest, Romania;Department of Biochemistry, Faculty of Dental Medicine, University of Medicine and Pharmacy, Carol Davila, Blvd-ul Eroii Sanitari, No 8, Bucharest, Romania;Victor Babes National Institute of Pathology, Biochemistry-Proteomics Department, Blv. Splaiul Independenţei nr. 99 - 101, Bucharest, Romania;Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, Pta 1 Decembrie nr.10, Oradea, Romania;Department of Functional Sciences-Immunology, Victor Babes University of Medicine and Pharmacy, Pta Eftimie Murgu nr.2, 300041, Timisoara, Romania;Department of Odontotherapy and Endodontics, Victor Babes University of Medicine and Pharmacy, Bv. Revolutiei 1989, 9, 300041, Timisoara, Romania;Department of Periodontology, Victor Babes University of Medicine and Pharmacy, Bv. Revolutiei 1989, Nr. 9, 300041, Timisoara, Romania;

关键词: Keratinocytes;    T-lymphocytes;    Oral mucosa;    Collagen matrix;   

DOI  :  10.1186/s12903-016-0240-x

 received in 2015-09-14, accepted in 2016-06-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe aim of the present study is to analyze the behavior of selected populations of oral keratinocytes and T-lymphocytes, responsible for re-constructing and maintaining the oral epithelial tissue architecture, following augmentation of the keratinized oral mucosa using a 3D-collagen matrix.MethodsDifferent groups of oral keratinocytes were isolated from biopsies harvested from 3 patients before the surgical procedure, as well as 7 and 14 days after the augmentation procedure. T-lymphocytes were isolated from peripheral blood at same timepoints. Keratinocytes were characterized for stem and differentiation markers, such as p63, cytokeratin 10 and 14, and in vitro parameters, such as cell viability, cell size and colony-forming efficiency. T-lymphocytes were analyzed for viability and the expression of various cluster of differentiation markers. The methods included magnetic separation of cell populations, immunofluorescence, flow cytometry, and histology of oral biopsies.ResultsBoth at 7 and 14 days, the majority of cells that repopulate the matrix were actively proliferating/progenitor oral keratinocytes with the phenotype integrin alfa6beta4 + CD71+. These cells display in vitro characteristics similar to the progenitor cells analyzed before the matrix placement. T-lymphocytes expressed CD8 and CD69 markers, while CD25 was absent.ConclusionThe study shows that two weeks after the collagen membrane placement, the healing process appeared to be histologically complete, with no abnormal immune response induced by the matrix, however, with a higher than usual content of active proliferating cells, the majority of keratinocytes being characterized as transit amplifying cells.

【 授权许可】

CC BY   
© The Author(s). 2016