Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice

Reproductive Biology and Endocrinology

Research

Agnès Noel¹  Carine Munaut¹  Jean-Michel Foidart¹  Maïté Fransolet¹  Silvia Blacher¹  Laurie Henry²  Michelle Nisolle²  Soraya Labied²  Nathalie Kirschvink³ 

[1] Laboratory of Tumor and Development Biology, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA-R), University of Liège (B23) Sart-Tilman, B-4000, Liège, Belgium;Laboratory of Tumor and Development Biology, Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA-R), University of Liège (B23) Sart-Tilman, B-4000, Liège, Belgium;Department of Gynecology, University of Liège, Boulevard du XIIème de Ligne, B-4000, Liège, Belgium;Veterinary Integrated Research Unit, University of Namur, Rue de Bruxelles 61, B-5000, Namur, Belgium;

关键词: Collagen matrix;    VEGF165;    Fertility preservation;    Xenotransplantation and angiogenesis;   

DOI  :  10.1186/s12958-015-0015-2

 received in 2014-10-23, accepted in 2015-02-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement.MethodsFrozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies.ResultsWe found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3 days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3 weeks, the density of murine vessels was significantly higher in the VEGF165 group.ConclusionThe encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.

【 授权许可】

Unknown   
© Henry et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.