Use of Biologically Active 3D Matrix for Extensive Skin Defect Treatment in Veterinary Practice: Case Report

Frontiers in Veterinary Science

Margarita N. Zhuravleva¹  Elena Yu Zakirova¹  Albert A. Rizvanov¹  Ekaterina E. Garanina¹  Catrin S. Rutland²  Dmitry V. Shalimov³ 

[1] Department of Exploratory Research, Scientific and Educational Center of Pharmaceutics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia;Faculty of Medicine, School of Veterinary Medicine and Science, University of Nottingham, Nottingham, United Kingdom;Veterinary Clinic “6 Elephants”, Kazan, Russia;

关键词: mesenchymal stem cells;    fibrin glue;    3D matrix;    VEGF165;    FGF2;   

DOI  :  10.3389/fvets.2019.00076

来源: DOAJ

【 摘 要 】

Objectives: Large full-thickness skin defects represent a serious veterinary problem.Methods: We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs.Results:In vitro studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes in vitro. Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans.

【 授权许可】

Unknown