Whole-cell biocatalysts by design

Microbial Cell Factories

Review

Baixue Lin¹  Yong Tao¹ 

[1] CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, People’s Republic of China;

关键词: Whole-cell biocatalysis;    Design;    Optimization;    Biosynthetic pathway;    Cofactor balance;    Metabolic engineering;    Synthetic biology;   

DOI  :  10.1186/s12934-017-0724-7

 received in 2016-12-28, accepted in 2017-06-08,  发布年份 2017

来源: Springer

【 摘 要 】

Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals.

【 授权许可】

CC BY   
© The Author(s) 2017