Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay

Malaria Journal

Research

Wichai Satimai¹  Soklyda Chann²  Mark M Fukuda²  Kritsanai Yingyuen²  Kurt Schaecher²  Stuart D Tyner²  Piyaporn Saingam²  Chanthap Lon²  David L Saunders²  Sabaithip Sriwichai²  Delia Bethell²  Panjaporn Chaichana²  Suwanna Chaorattanakawee²  Ans Timmermans²  Douglas S Walsh²  Youry Se²  Nillawan Buathong²  Wiriya Rutvisuttinunt²  Siratchana Sundrakes²  Harald Noedl³  Doung Socheat⁴  Darapiseth Sea⁴ 

[1] Bureau of Vector-Borne and Zoonotic Diseases, Thailand Ministry of Public Health, Nonthaburi, Thailand;Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences (USAMC-AFRIMS), Bangkok, Thailand;Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090, Vienna, Austria;The National Center for Parasitology, Entomology and Malaria Control (CNM), Phnom Penh, Cambodia;

关键词: Cambodia;    malaria;    Plasmodium falciparum;    HRP-2;    Anti-malarial drugs;    Drug resistance;   

DOI  :  10.1186/1475-2875-11-198

 received in 2012-04-02, accepted in 2012-06-13,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundIn vitro drug susceptibility assay of Plasmodium falciparum field isolates processed “immediate ex vivo” (IEV), without culture adaption, and tested using histidine-rich protein-2 (HRP-2) detection as an assay, is an expedient way to track drug resistance.MethodsFrom 2005 to 2010, a HRP-2 in vitro assay assessed 451 P. falciparum field isolates obtained from subjects with malaria in western and northern Cambodia, and eastern Thailand, processed IEV, for 50% inhibitory concentrations (IC50) against seven anti-malarial drugs, including artesunate (AS), dihydroartemisinin (DHA), and piperaquine.ResultsIn western Cambodia, from 2006 to 2010, geometric mean (GM) IC50 values for chloroquine, mefloquine, quinine, AS, DHA, and lumefantrine increased. In northern Cambodia, from 2009–2010, GM IC50 values for most drugs approximated the highest western Cambodia GM IC50 values in 2009 or 2010.ConclusionsWestern Cambodia is associated with sustained reductions in anti-malarial drug susceptibility, including the artemisinins, with possible emergence, or spread, to northern Cambodia. This potential public health crisis supports continued in vitro drug IC50 monitoring of P. falciparum isolates at key locations in the region.

【 授权许可】

Unknown   
© Tyner et al. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.