Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay

Malaria Journal

关键词: Cambodia;    malaria;    Plasmodium falciparum;    HRP-2;    Anti-malarial drugs;    Drug resistance;   

DOI  :  10.1186/1475-2875-11-198

来源: DOAJ

【 摘 要 】

Abstract

Background

In vitro drug susceptibility assay of Plasmodium falciparum field isolates processed “immediate ex vivo” (IEV), without culture adaption, and tested using histidine-rich protein-2 (HRP-2) detection as an assay, is an expedient way to track drug resistance.

Methods

From 2005 to 2010, a HRP-2 in vitro assay assessed 451 P. falciparum field isolates obtained from subjects with malaria in western and northern Cambodia, and eastern Thailand, processed IEV, for 50% inhibitory concentrations (IC₅₀) against seven anti-malarial drugs, including artesunate (AS), dihydroartemisinin (DHA), and piperaquine.

Results

In western Cambodia, from 2006 to 2010, geometric mean (GM) IC₅₀ values for chloroquine, mefloquine, quinine, AS, DHA, and lumefantrine increased. In northern Cambodia, from 2009–2010, GM IC₅₀ values for most drugs approximated the highest western Cambodia GM IC₅₀ values in 2009 or 2010.

Conclusions

Western Cambodia is associated with sustained reductions in anti-malarial drug susceptibility, including the artemisinins, with possible emergence, or spread, to northern Cambodia. This potential public health crisis supports continued in vitro drug IC₅₀ monitoring of P. falciparum isolates at key locations in the region.

【 授权许可】

Unknown