Species and genotype diversity of Plasmodium in malaria patients from Gabon analysed by next generation sequencing

Malaria Journal

Research

Ghyslain Mombo-Ngoma¹  Sabine Bélard²  Fanny Joanny³  Annika Lang³  Thomas Engleitner⁴  Albert Lalremruata⁵  Benjamin Mordmüller⁶  Jana Held⁶  Peter G. Kremsner⁶  Michael Ramharter⁷  Sankarganesh Jeyaraj⁸ 

[1] Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon;Department of Pediatric Pneumology and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany;Berlin Institute of Health, Berlin, Germany;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, Munich, Germany;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;German Centre for Infection Research, partner site Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;German Centre for Infection Research, partner site Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;German Centre for Infection Research, partner site Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon;Department of Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria;Institut für Tropenmedizin, Eberhard Karls Universität Tübingen, Wilhelmstraße 27, 72074, Tübingen, Germany;PSG Institute of Advanced Studies, 641 004, Coimbatore, India;

关键词: Plasmodium malariae;    Plasmodium falciparum;    Plasmodium ovale wallikeri;    Plasmodium ovale curtisi;    Next-generation sequencing;    Amplicon;    Metagenomics;   

DOI  :  10.1186/s12936-017-2044-0

 received in 2017-06-14, accepted in 2017-09-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSix Plasmodium species are known to naturally infect humans. Mixed species infections occur regularly but morphological discrimination by microscopy is difficult and multiplicity of infection (MOI) can only be evaluated by molecular methods. This study investigated the complexity of Plasmodium infections in patients treated for microscopically detected non-falciparum or mixed species malaria in Gabon.MethodsUltra-deep sequencing of nucleus (18S rRNA), mitochondrion, and apicoplast encoded genes was used to evaluate Plasmodium species diversity and MOI in 46 symptomatic Gabonese patients with microscopically diagnosed non-falciparum or mixed species malaria.ResultsDeep sequencing revealed a large complexity of coinfections in patients with uncomplicated malaria, both on species and genotype levels. Mixed infections involved up to four parasite species (Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale curtisi, and P. ovale wallikeri). Multiple genotypes from each species were determined from the asexual 18S rRNA gene. 17 of 46 samples (37%) harboured multiple genotypes of at least one Plasmodium species. The number of genotypes per sample (MOI) was highest in P. malariae (n = 4), followed by P. ovale curtisi (n = 3), P. ovale wallikeri (n = 3), and P. falciparum (n = 2). The highest combined genotype complexity in samples that contained mixed-species infections was seven.ConclusionsUltra-deep sequencing showed an unexpected breadth of Plasmodium species and within species diversity in clinical samples. MOI of P. ovale curtisi, P. ovale wallikeri and P. malariae infections were higher than anticipated and contribute significantly to the burden of malaria in Gabon.

【 授权许可】

CC BY   
© The Author(s) 2017