Evidence of non-Plasmodium falciparum malaria infection in Kédougou, Sénégal

Malaria Journal

Research

Katelyn Durfee¹  Rachel F. Daniels²  Dyann F. Wirth²  Sarah K. Volkman³  Awa Bineta Deme⁴  Baba Dieye⁴  Daouda Ndiaye⁵  Jules F. Gomis⁵  Aida S. Badiane⁵  Julie I. Thwing⁶  Yaye Die Ndiaye⁷  Fatou B. Fall⁷  Mady Ba⁷  Medoune Ndiop⁷ 

[1] Department of Immunology and Infectious Disease, Harvard T.H. Chan School of Public Health, Boston, MA, USA;Department of Immunology and Infectious Disease, Harvard T.H. Chan School of Public Health, Boston, MA, USA;Infectious Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA, USA;Department of Immunology and Infectious Disease, Harvard T.H. Chan School of Public Health, Boston, MA, USA;Infectious Disease Initiative, Broad Institute of MIT and Harvard, Cambridge, MA, USA;School of Nursing and Health Sciences, Simmons College, Boston, MA, USA;Department of Parasitology and Mycology, Cheikh Anta Diop University, Dakar, Senegal;Department of Parasitology and Mycology, Cheikh Anta Diop University, Dakar, Senegal;Laboratory of Parasitology and Mycology, Cheikh Anta Diop University/Le Dantec Hospital, Dakar, Senegal;Malaria Branch, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA;Malaria Branch, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, USA;President’s Malaria Initiative, Dakar, Senegal;National Malaria Control Programme, Dakar, Senegal;

关键词: Plasmodium falciparum;    Plasmodium ovale curtisi;    Plasmodium ovale wallikeri;    Plasmodium malariae;    Rapid diagnostic test;   

DOI  :  10.1186/s12936-016-1661-3

 received in 2016-10-14, accepted in 2016-12-16,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundExpanded malaria control efforts in Sénégal have resulted in increased use of rapid diagnostic tests (RDT) to identify the primary disease-causing Plasmodium species, Plasmodium falciparum. However, the type of RDT utilized in Sénégal does not detect other malaria-causing species such as Plasmodium ovale spp., Plasmodium malariae, or Plasmodium vivax. Consequently, there is a lack of information about the frequency and types of malaria infections occurring in Sénégal. This study set out to better determine whether species other than P. falciparum were evident among patients evaluated for possible malaria infection in Kédougou, Sénégal.MethodsReal-time polymerase chain reaction speciation assays for P. vivax, P. ovale spp., and P. malariae were developed and validated by sequencing and DNA extracted from 475 Plasmodium falciparum-specific HRP2-based RDT collected between 2013 and 2014 from a facility-based sample of symptomatic patients from two health clinics in Kédougou, a hyper-endemic region in southeastern Sénégal, were analysed.ResultsPlasmodium malariae (n = 3) and P. ovale wallikeri (n = 2) were observed as co-infections with P. falciparum among patients with positive RDT results (n = 187), including one patient positive for all three species. Among 288 negative RDT samples, samples positive for P. falciparum (n = 24), P. ovale curtisi (n = 3), P. ovale wallikeri (n = 1), and P. malariae (n = 3) were identified, corresponding to a non-falciparum positivity rate of 2.5%.ConclusionsThese findings emphasize the limitations of the RDT used for malaria diagnosis and demonstrate that non-P. falciparum malaria infections occur in Sénégal. Current RDT used for routine clinical diagnosis do not necessarily provide an accurate reflection of malaria transmission in Kédougou, Sénégal, and more sensitive and specific methods are required for diagnosis and patient care, as well as surveillance and elimination activities. These findings have implications for other malaria endemic settings where species besides P. falciparum may be transmitted and overlooked by control or elimination activities.

【 授权许可】

CC BY   
© The Author(s) 2017