¹H NMR-based metabolic profiling of human rectal cancer tissue

Molecular Cancer

Research

Pengchi Deng¹  Bin Zhou²  Zongguang Zhou²  Yuan Li³  Xiaoni Shao⁴  Huijuan Wang⁴  Hailong Zhang⁴  Jie Chen⁴  Tianming Wu⁴  Pu Xiang⁴  Ying-Lan Zhao⁴  Jing Hu⁴  Wenjie Lu⁴  Liang Wang⁴  Jun Zou⁴ 

[1] Analytical & Testing Center, Sichuan University, 610041, Chengdu, China;Department of Gastrointestinal surgery, West China Hospital, West China Medical School, Sichuan University, 610041, Chengdu, China;Department of Pediatric Surgery, West China Hospital, West China Medical School, Sichuan University, 610041, Chengdu, China;State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, 17#, 3rd Section, Ren min South Road, 610041, Chengdu, China;

关键词: Rectal Cancer;    Taurine;    Rectal Cancer Patient;    Partial Little Square Discriminant Analysis;    Nuclear Magnetic Resonance Data;   

DOI  :  10.1186/1476-4598-12-121

 received in 2013-07-03, accepted in 2013-09-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundRectal cancer is one of the most prevalent tumor types. Understanding the metabolic profile of rectal cancer is important for developing therapeutic approaches and molecular diagnosis.MethodsHere, we report a metabonomics profiling of tissue samples on a large cohort of human rectal cancer subjects (n = 127) and normal controls (n = 43) using 1H nuclear magnetic resonance (1H NMR) based metabonomics assay, which is a highly sensitive and non-destructive method for the biomarker identification in biological systems. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal projection to latent structure with discriminant analysis (OPLS-DA) were applied to analyze the 1H-NMR profiling data to identify the distinguishing metabolites of rectal cancer.ResultsExcellent separation was obtained and distinguishing metabolites were observed among the different stages of rectal cancer tissues (stage I = 35; stage II = 37; stage III = 37 and stage IV = 18) and normal controls. A total of 38 differential metabolites were identified, 16 of which were closely correlated with the stage of rectal cancer. The up-regulation of 10 metabolites, including lactate, threonine, acetate, glutathione, uracil, succinate, serine, formate, lysine and tyrosine, were detected in the cancer tissues. On the other hand, 6 metabolites, including myo-inositol, taurine, phosphocreatine, creatine, betaine and dimethylglycine were decreased in cancer tissues. These modified metabolites revealed disturbance of energy, amino acids, ketone body and choline metabolism, which may be correlated with the progression of human rectal cancer.ConclusionOur findings firstly identify the distinguishing metabolites in different stages of rectal cancer tissues, indicating possibility of the attribution of metabolites disturbance to the progression of rectal cancer. The altered metabolites may be as potential biomarkers, which would provide a promising molecular diagnostic approach for clinical diagnosis of human rectal cancer. The role and underlying mechanism of metabolites in rectal cancer progression are worth being further investigated.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.