Enzyme-mediated formulation of stable elliptical silver nanoparticles tested against clinical pathogens and MDR bacteria and development of antimicrobial surgical thread

Annals of Clinical Microbiology and Antimicrobials

Research

Pravin Dudhagara¹  Chintan Bhagat¹  Pragya Shrestha²  Rupak Thapa²  Suvash Awal² 

[1] Department of Biosciences (UGC-SAP-DRS-II), Veer Narmad South Gujarat University, 395007, Surat, Gujarat, India;Department of Biotechnology, Veer Narmad South Gujarat University, 395007, Surat, Gujarat, India;

关键词: Silver nanoparticles (AgNPs);    Antimicrobial activity;    Multi-drug resistant (MDR);    Surgical thread;   

DOI  :  10.1186/s12941-017-0216-y

 received in 2017-04-04, accepted in 2017-05-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSilver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV–Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD).ResultsCharacterization studies suggest the synthesis of elliptical, stable and crystalline AgNPs with an average size of 38.26 ± 0.4 nm calculated using TEM. The XRD pattern revealed the face-centered-cubic (fcc) form of metallic silver. Good shape integrity and dispersion of AgNPs after 1 year of incubation confirmed their stability. AgNPs were exibited the antimicrobial property against ten pathogenic bacteria, three molds and one yeast. The AgNPs also revealed remarkable antimicrobial activity against three MDR strains i.e. Extended spectrum beta-lactamase positive Escherichia coli, Staphylococcus aureus (MRSA) and Teicoplanin resistant Streptococcus Pneumoniae. The AgNPs coated surgical threads (suture) were revealed the remarkble antibacterial activity against three MDR strains. This is the first report to synthesize antimicrobial elliptical AgNPs using enzymes.Conclusion The results suggest the possibilities to develop the nanoparticles coated antimicrobial medical fabric to combat against MDR infection.

【 授权许可】

CC BY   
© The Author(s) 2017