Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China

BMC Veterinary Research

Research Article

Feng Gao¹  Deguang Song¹  Kui Zhao¹  Zhangyong Ning²  Daniel L Rock³  Wei Li⁴  Xiaoqing Liao⁴  Wenbo Hao⁴  Ming Li⁴  Shuhong Luo⁵ 

[1] College of Animal Science and Veterinary Medicine, Jilin University, 130062, Changchun, China;College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, People’s Republic of China;Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Champaign-Urbana, S. Lincoln Avenue, 2001, 61802, Urbana, IL, USA;Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Champaign-Urbana, S. Lincoln Avenue, 2001, 61802, Urbana, IL, USA;Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, N. Guangzhou Avenue, 1838, 510515, Guangzhou, People’s Republic of China;Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, N. Guangzhou Avenue, 1838, 510515, Guangzhou, People’s Republic of China;College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, People’s Republic of China;

关键词: Parapoxvirus;    Orf virus;    Phylogenetic analysis;    Sheep;    Zoonosis;    nMDS;   

DOI  :  10.1186/1746-6148-8-229

 received in 2012-05-21, accepted in 2012-10-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundOrf is a zoonotic and epitheliotrophic contagious disease that mainly affects sheep, goats, wild ruminants, and humans with a worldwide distribution. To date, there is little information on the characterization of ORFV strains that are endemic in Mainland China. In addition, the relationship between the severity of disease and the molecular profile of ORFV strains has not been fully elucidated.ResultsFrom the recent outbreak of a sheep herd in Nongan, northeast of China, the novel orf virus (ORFV) strain NA1/11 was successfully isolated. Western blot analysis indicated that the NA1/11 strain cross reacts with monoclonal antibody A3 and infected sheep ORFV antiserum. The purified virions revealed the typical ovoid shape when observed by atomic force microscopy. To determine the genetic characteristics of the NA1/11 strain, the sequences of ORFV011 (B2L), ORFV059 (F1L), ORFV109, ORFV110 and ORFv132 (VEGF) genes were amplified and compared with reference parapoxvirus strains. Non-metric multidimensional scaling (nMDS) was performed to analyze the nucleotide similarities between different ORFV strains.ConclusionsPhylogenetic analysis based on ORFV 011 nucleotide sequences showed that the NA1/11strain was closely related to Xinjiang and Gansu strains. ORFV110 and ORFV132 genes are highly variable. The results revealed that precise phylogenetic analysis might provide evidence for genetic variation and movement of circulating ORFV strains in Northeast China. In addition, nMDS analysis showed that geographic isolation and animal host are likely major factors resulting in genetic differences between ORFV strains.

【 授权许可】

Unknown   
© Li et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.