Isolation and phylogenetic analysis of orf virus from the sheep herd outbreak in northeast China

BMC Veterinary Research

Shuhong Luo⁴  Daniel L Rock²  Ming Li¹  Xiaoqing Liao¹  Kui Zhao³  Feng Gao³  Deguang Song³  Wenbo Hao¹  Zhangyong Ning²  Wei Li¹ 

[1] Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, N. Guangzhou Avenue, 1838, Guangzhou, 510515, People’s Republic of China;Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Champaign-Urbana, S. Lincoln Avenue, 2001, Urbana, IL 61802, USA;College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China;College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

关键词: nMDS;    Zoonosis;    Sheep;    Phylogenetic analysis;    Orf virus;    Parapoxvirus;   

Others  :  1119658
DOI  :  10.1186/1746-6148-8-229

 received in 2012-05-21, accepted in 2012-10-16,  发布年份 2012

【 摘 要 】

Background

Orf is a zoonotic and epitheliotrophic contagious disease that mainly affects sheep, goats, wild ruminants, and humans with a worldwide distribution. To date, there is little information on the characterization of ORFV strains that are endemic in Mainland China. In addition, the relationship between the severity of disease and the molecular profile of ORFV strains has not been fully elucidated.

Results

From the recent outbreak of a sheep herd in Nongan, northeast of China, the novel orf virus (ORFV) strain NA1/11 was successfully isolated. Western blot analysis indicated that the NA1/11 strain cross reacts with monoclonal antibody A3 and infected sheep ORFV antiserum. The purified virions revealed the typical ovoid shape when observed by atomic force microscopy. To determine the genetic characteristics of the NA1/11 strain, the sequences of ORFV011 (B2L), ORFV059 (F1L), ORFV109, ORFV110 and ORFv132 (VEGF) genes were amplified and compared with reference parapoxvirus strains. Non-metric multidimensional scaling (nMDS) was performed to analyze the nucleotide similarities between different ORFV strains.

Conclusions

Phylogenetic analysis based on ORFV 011 nucleotide sequences showed that the NA1/11strain was closely related to Xinjiang and Gansu strains. ORFV110 and ORFV132 genes are highly variable. The results revealed that precise phylogenetic analysis might provide evidence for genetic variation and movement of circulating ORFV strains in Northeast China. In addition, nMDS analysis showed that geographic isolation and animal host are likely major factors resulting in genetic differences between ORFV strains.

【 授权许可】

   
2012 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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