Molecular Reidentification of Endophytic Fungus LBKURCC40 Isolated from Dahlia's Tuber (Dahlia Variabilis) Using the Sequence of ITS rDNA

4th International Conference on Science, Technology and Interdisciplinary Research

工业技术（总论）;自然科学（总论）

Suraya, Nabella^1 ; Piska, Finna^1 ; Pratiwi, Nova Wahyu^2 ; Ardhi, Aulia^3 ; Saryono^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau, Indonesia^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau, Indonesia^2

Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Indonesia^3

关键词: dahlia;    Endophytic fungi;    Molecular identification;    Phylogenetic analysis;    Re identifications;    Trichoderma;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/532/1/012021/pdf
DOI  :  10.1088/1757-899X/532/1/012021

来源: IOP

【 摘 要 】

Endophytic fungus LBKURCC40 isolated from dahlia tubers (Dahlia variabilis) was morphologically and molecularly identified. Molecular identification was carried out using ITS4 and ITS5 primers, furthermore, the homology level of LBKURCC40 isolates was checked using BLAST program and the suitability of BLAST results was tested with the TrichOKEY program. The results of showed that LBKURCC40 isolates were identified as Trichoderma asperellum with a homology level of 99%. Phylogenetic analysis was carried out with N-J tree bootstrap test with 10,000 repetitions, and LBKURCC40 isolate was obtained to have the closest kinship to Trichoderma asperellum strain Tr266B.