Majallah-i ḥifāẓat-i giyāhān | |
Molecular Identification of Turnip Mosaic virus (TuMV) in Hoary Mustard (Herschfeldia incana) From Iran | |
S. Tarighi1  M. A. Sabokkhiz1  F. Shahriari Ahmadi1  B. Jafarpour1  M.R. Safarnejad2  | |
[1] Ferdowsi University of Mashhad;Iranian Research Institute of Plant Protection; | |
关键词: Hirschfeldia incana (L.) Lagr.-Foss.; Molecular identification; Phylogenetic analysis; Turnip mosaic virus (TuMV); | |
DOI : 10.22067/jpp.v29i2.31558 | |
来源: DOAJ |
【 摘 要 】
Turnip mosaic virus (TuMV) is amember of the Potyvirus genus within the Potyviridae family and is one the most important viruses infecting Brassicaceae plants. In April 2012, suspicious symptoms of a viral disease such as mosaic, stunting and malformation were observed on Herschfeldia incana. The collected samples were tested using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers corresponding toTuMV coat protein gene. Amplified fragment (986bp) was first purified and then directly sequenced. Analysis of its CP nucleotide and amino acid sequence revealed 85.42-89.58 % and 91.64-95.12% similarity to those of 31 TuMV isolates from other countries respectively.Phylogenetic tree was constructedby MEGA6 software using neighbor joining method. The results showed thatthe TuMV isolate and 3 Iranian isolateshave been clustered into the basal-B group.
【 授权许可】
Unknown