Molecular Identification of Turnip Mosaic virus (TuMV) in Hoary Mustard (Herschfeldia incana) From Iran

Majallah-i ḥifāẓat-i giyāhān

S. Tarighi¹  M. A. Sabokkhiz¹  F. Shahriari Ahmadi¹  B. Jafarpour¹  M.R. Safarnejad² 

[1] Ferdowsi University of Mashhad;Iranian Research Institute of Plant Protection;

关键词: Hirschfeldia incana (L.) Lagr.-Foss.;    Molecular identification;    Phylogenetic analysis;    Turnip mosaic virus (TuMV);   

DOI  :  10.22067/jpp.v29i2.31558

来源: DOAJ

【 摘 要 】

Turnip mosaic virus (TuMV) is amember of the Potyvirus genus within the Potyviridae family and is one the most important viruses infecting Brassicaceae plants. In April 2012, suspicious symptoms of a viral disease such as mosaic, stunting and malformation were observed on Herschfeldia incana. The collected samples were tested using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers corresponding toTuMV coat protein gene. Amplified fragment (986bp) was first purified and then directly sequenced. Analysis of its CP nucleotide and amino acid sequence revealed 85.42-89.58 % and 91.64-95.12% similarity to those of 31 TuMV isolates from other countries respectively.Phylogenetic tree was constructedby MEGA6 software using neighbor joining method. The results showed thatthe TuMV isolate and 3 Iranian isolateshave been clustered into the basal-B group.

【 授权许可】

Unknown