DNA methylation-regulated LINC02587 inhibits ferroptosis and promotes the progression of glioma cells through the CoQ-FSP1 pathway

BMC Cancer

Research

Fanchen Wang¹  Yan Yan²  Zhengang Wang³  Yang Cui⁴  Lixia Xu⁵  Xiaoguang Tong⁶  Hua Yan⁶ 

[1] Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Cancer Molecular Diagnostics Core, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, 300060, Tianjin, China;Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Clinical Laboratory, Tianjin Huanhu Hospital, 300350, Tianjin, China;Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Department of Neurosurgery, Affiliated Hospital of Weifang Medical University, 261000, Weifang, Shan Dong, China;Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Department of Neurosurgery, Hebei Yanda Hospital, Langfang, He Bei, China;Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, 300350, Tianjin, China;Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, 300350, Tianjin, China;Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, 300350, Tianjin, China;Department of Neurosurgery, Tianjin Huanhu Hospital, 300350, Tianjin, China;

关键词: Glioma;    LncRNA;    LINC02587;    Methylation;    Ferroptosis;    RNA-seq;    CoQ-FSP1;   

DOI  :  10.1186/s12885-023-11502-0

 received in 2023-01-02, accepted in 2023-10-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLong noncoding RNAs (lncRNAs) are considered key players in the formation and development of tumors. Herein, Gene Expression Profiling Interactive Analysis (GEPIA) was employed as a bioinformatics technology. LINC02587 is differentially expressed in bladder urothelial cancer, glioblastoma, lung adenocarcinoma, lung SCC, melanoma, and other tumor tissue and cells. However, its impact on the emergence of glioma and its mechanism is remaining elusive.MethodsSome of the in vitro assays employed in this study were the CCK-8 / Annexin-V / Transwell assays, colony formation, and wound healing, together with Western blot (WB) evaluation. MSP / BSP assays were employed for assessing the CpG island’s methylation status in the LINC02587 promoter. Through transcriptome, ferroptosis-related experiments, and WB evaluation, it was confirmed that LINC02587 is correlated with the regulation of ferroptosis in tumor cells, and CoQ-Fsp1 is one of its regulatory pathways. Moreover, the underlined in-vitro results were further validated by in-vivo studies.ResultsThe current study shows that the promoter sequence of LINC02587 is regulated by methylation. The silencing of LINC02587 can inhibit cellular proliferative, migrative, and invasive properties, and induce ferroptosis within gliomas through the CoQ-FSP1 pathway.ConclusionsLINC02587 is likely to be a novel drug target in treating glioma.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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