Sequence analysis of genes mediating extended-spectrum beta-lactamase (ESBL) production in isolates of Enterobacteriaceae in a Lagos Teaching Hospital, Nigeria

BMC Infectious Diseases

Research Article

Muhabat Adeola Raji¹  Wafaa Jamal²  Vincent Olubunmi Rotimi²  Omoh Ojemeh³ 

[1] Department of Medical Microbiology and Parasitology, Lagos State University College of Medicine/Lagos State University Teaching Hospital, Ikeja, Nigeria;Microbiology Laboratory, BT Health and Diagnostic Centre, Lagos State University Teaching Hospital, Ikeja, Nigeria;Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait;Microbiology Laboratory, BT Health and Diagnostic Centre, Lagos State University Teaching Hospital, Ikeja, Nigeria;

关键词: Multidrug resistance;    ESBL;    Enterobacteriaceae;    Southwest Nigeria;    CTX-M-15;   

DOI  :  10.1186/s12879-015-1005-x

 received in 2014-08-15, accepted in 2015-06-30,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundExtended-spectrum β-lactamases (ESBLs) in Gram-negative organisms is now a major concern in Enterobacteriaceae worldwide. This study determined a point-prevalence and genetic profiles of ESBL-producing isolates among members of the family Enterobacteriaceae in Lagos State University Teaching Hospital Ikeja, Nigeria.MethodsConsecutive non-repetitive invasive multidrug-resistant isolates of the family Enterobacteriaceae obtained over a period of 1 month (October 2011) were studied. The isolates were identified using VITEK-2/VITEK MS Systems. Susceptibility testing was performed using E test technique; results were interpreted according to the criteria recommended by the Clinical and Laboratory Standards Institute (CLSI, 2012). ESBL production was detected by E test ESBL method and confirmed by polymerase chain reaction (PCR).ResultsDuring the one-month study period, 38 isolates with ESBL phenotypic characteristics were identified and confirmed by PCR. Of these, 21 (55.3 %) were E. coli, 12 (31.6 %) K. pneumoniae, 3 (7.9 %) Proteus spp., 1 (2.6 %) each M. morganii and C. freundii. Thirty (79 %) harbored blaCTX-M genes. Sequence analysis revealed that they were all blaCTX-M-15 genes. Twenty-nine (96.7 %) of these, also harbored blaTEM genes simultaneously. All the CTX-M-15-producing isolates carried insertion sequence blaISEcP1 upstream of blaCTX-M-15 genes. The E. coli isolates were genetically heterogeneous, while the K. pneumoniae had 98 % homology.ConclusionsOur point-prevalence surveillance study revealed a high prevalence of Enterobacteriaceae isolates harboring blaCTX-M-15 in the Hospital. Urgent implementation of antibiotic stewardship and other preventive strategies are necessary at this time in our hospital.

【 授权许可】

CC BY   
© Raji et al. 2015