Lipid peroxidation and antioxidant enzymes activity in Plasmodium vivax malaria patients evolving with cholestatic jaundice

Malaria Journal

Research

Emerson S Lima¹  Camila Fabbri¹  Pritesh Lalwani²  Márcia AA Alexandre³  Marcus VG Lacerda³  Gisely C Melo³  Belisa ML Magalhães³  Rita de Cássia Mascarenhas-Netto⁴ 

[1] Faculty of Pharmaceutical Sciences, Universidade Federal do Amazonas, AM 69010-300, Manaus, Brazil;Faculty of Pharmaceutical Sciences, Universidade Federal do Amazonas, AM 69010-300, Manaus, Brazil;Institute of Medical Virology, Charité – Universitätsmedizin Berlin, D-10117, Berlin, Germany;Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, AM 69040-000, Manaus, Brazil;Universidade do Estado do Amazonas, AM 69040-000, Manaus, Brazil;Institute of Biochemistry and Genetics, Universidade Federal de Uberlândia, MG 38400-902, Minas, Brazil;

关键词: Malaria;    Plasmodium vivax;    Antioxidant enzymes;    Oxidative stress;    Jaundice;    Hyperbilirubinaemia;   

DOI  :  10.1186/1475-2875-12-315

 received in 2013-02-18, accepted in 2013-09-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPlasmodium vivax infection has been considered a benign and self-limiting disease, however, recent studies highlight the association between vivax malaria and life-threatening manifestations. Increase in reactive oxygen species has already been described in vivax malaria, as a result of the increased metabolic rate triggered by the multiplying parasite, and large quantities of toxic redox-active byproducts generated. The present study aimed to study the oxidative stress responses in patients infected with P. vivax, who developed jaundice (hyperbilirubinaemia) in the course of the disease, a common clinical complication related to this species.MethodsAn evaluation of the lipid peroxidation and antioxidant enzymes profile was performed in 28 healthy individuals and compared with P. vivax infected patients with jaundice, i.e., bilirubin < 51.3 μmol/L (8 patients) or without jaundice (34 patients), on day 1 (D1) and day 14 (D14) after anti-malarial therapy.ResultsHyperbilirubinaemia was more frequent among women and patients experiencing their first malarial infection, and lower haemoglobin and higher lactate dehydrogenase levels were observed in this group. Malondialdehyde levels and activity of celuroplasmin and glutathione reductase were increased in the plasma from patients with P. vivax with jaundice compared to the control group on D1. However, the activity of thioredoxin reductase was decreased. The enzymes glutathione reductase, thioredoxin reductase, thiols and malondialdehyde also differed between jaundiced versus non-jaundiced patients. On D14 jaundice and parasitaemia had resolved and oxidative stress biomarkers were very similar to the control group.ConclusionCholestatic hyperbilirubinaemia in vivax malaria cannot be totally disassociated from malaria-related haemolysis. However, significant increase of lipid peroxidation markers and changes in antioxidant enzymes in patients with P. vivax-related jaundice was observed. These results suggest oxidative processes contributing to malaria pathogenesis, what may be useful information for future anti-oxidant therapeutical interventions in these patients.

【 授权许可】

Unknown   
© Fabbri et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.