Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa

Journal of Nanobiotechnology

Research

Liew Jian Ping¹  Rajkumar Durairaj¹  Jamal Hussaini²  Zaini Mohd-Zain²  Athirah Nur Amirulhusni²  Navindra Kumari Palanisamy²  Nas Ferina² 

[1] Faculty of Engineering and Science, University Tunku Abdul Rahman Jalan Genting Kelang, 53300, Kuala Lumpur, Setapak, Malaysia;Faculty of Medicine, Universiti Teknologi MARA (UiTM), Jalan Hospital, Sg. Buloh, 47000, Selangor, Malaysia;

关键词: Antibacterial activity;    Silver nanoparticles;    Biofilm;    Inhibitory effect;    Pseudomonas aeruginosa;   

DOI  :  10.1186/1477-3155-12-2

 received in 2013-10-10, accepted in 2014-01-03,  发布年份 2014

来源: Springer

【 摘 要 】

Nanomedicine is now being introduced as a recent trend in the field of medicine. It has been documented that metal nanoparticles have antimicrobial effects for bacteria, fungi and viruses. Recent advances in technology has revived the use of silver nanoparticles in the medical field; treatment, diagnosis, monitoring and control of disease. It has been used since ancient times for treating wide range of illnesses. Bacterial cells adheres to surfaces and develop structures known as biofilms. These structures are natural survival strategy of the bacteria to invade the host. They are more tolerant to commonly used antimicrobial agents, thus being more difficult to be controlled. This leads to increase in severity of infection. In this study, we have investigated the effect of silver nanoparticles in the formation of biofilm in multidrug resistant strains of Pseudomonas aeruginosa. Observation showed that biofilm formation occurred at bacterial concentration of 106 cfu/ml for the sensitive strain of P. aeruginosa while in the resistant strain, the biofilm was evident at bacterial concentration of about 103 cfu/ml. The biofilm were then tested against various concentrations of silver nanoparticles to determine the inhibitory effect of the silver nanoparticles. In the sensitive strain, 20 μg/ml of silver nanoparticles inhibited the growth optimally at bacterial concentration of 104 cfu/ml with an inhibition rate of 67%. Similarly, silver nanoparticles inhibited the formation of biofilm in the resistant strain at an optimal bacterial concentration of 105 cfu/ml with an inhibition rate of 56%. Thus, silver nanoparticles could be used as a potential alternative therapy to reduce severity of disease due to P. aeruginosa infections.

【 授权许可】

CC BY   
© Palanisamy et al.; licensee BioMed Central Ltd. 2014