In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice

Microbial Cell Factories

Research

Janja Završnik¹  Miha Butinar¹  Boris Turk²  Aleš Berlec³  Borut Štrukelj⁴ 

[1] Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia;Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia;Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000, Ljubljana, Slovenia;Faculty of Chemistry and Chemical Technology, University of Ljubljana, 1000, Ljubljana, Slovenia;Department of Biotechnology, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia;Department of Biotechnology, Jožef Stefan Institute, Jamova 39, 1000, Ljubljana, Slovenia;Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia;

关键词: In vivo imaging;    Lactic acid bacteria;    Lactococcus lactis;    Lactobacillus plantarum;    Escherichia coli;    Infrared fluorescent protein;    Mice;   

DOI  :  10.1186/s12934-015-0376-4

 received in 2015-06-12, accepted in 2015-11-05,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn vivo imaging of orally administered lactic acid bacteria (LAB) and commensal bacteria in mice is shown to provide information on the spatial and temporal distribution of bacteria in the gastrointestinal tract. The bacteria can be detected and monitored using bioluminescence or near-infrared fluorescence.ResultsFluorescence imaging of bacteria was established by expressing the infrared fluorescent protein IRFP713 in Lactococcus lactis, Lactobacillus plantarum and Escherichia coli. All three bacterial species were monitored in live mice and no major differences in transit time were observed. Bacteria passed through the stomach and small intestine in 1 h and the majority were secreted from the large intestine after 6–8 h. Intestinal localization of bacteria was confirmed by imaging the isolated intestines and culturing the intestinal content. The use of fluorescence tomography for spatial localization of fluorescent bacteria has been established. The expression of an additional infrared fluorescent protein IRFP682 enabled concomitant detection of two bacterial populations in live mice.ConclusionsThe present work provides a methodological basis for future studies of probiotic and theranostic actions of LAB in mouse disease models.

【 授权许可】

CC BY   
© Berlec et al. 2015