The combined effect of erythropoietin and granulocyte macrophage colony stimulating factor on liver regeneration after major hepatectomy in rats

World Journal of Surgical Oncology

Research

Matrona Frangou¹  Agathi Kondi-Pafiti¹  Nikolaos Dafnios²  Aliki Tympa²  Theodosios Theodosopoulos²  George Fragulidis²  Ioannis Vassiliou²  Evangelos Lolis²  Constantinos Nastos²  Vassilios Smyrniotis² 

[1] Department of Pathology, Medical School, University of Athens, Aretaieion Hospital, 11528, Athens, Greece;Experimental Surgical Unit, 2nd Department of Surgery, Medical School, University of Athens, Aretaieion Hospital, 11528, Athens, Greece;

关键词: Proliferate Cell Nuclear Antigen;    Liver Regeneration;    Partial Hepatectomy;    Major Hepatectomy;    Proliferate Cell Nuclear Antigen Expression;   

DOI  :  10.1186/1477-7819-8-57

 received in 2010-01-04, accepted in 2010-07-07,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundThe liver presents a remarkable capacity for regeneration after hepatectomy but the exact mechanisms and mediators involved are not yet fully clarified. Erythropoietin (EPO) and Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) have been shown to promote liver regeneration after major hepatectomy.Aim of this experimental study is to compare the impact of exogenous administration of EPO, GM-CSF, as well as their combination on the promotion of liver regeneration after major hepatectomy.MethodsWistar rats were submitted to 70% major hepatectomy. The animals were assigned to 4 experimental groups: a control group (n = 21) that received normal saline, an EPO group (n = 21), that received EPO 500 IU/kg, a GM-CSF group (n = 21) that received 20 mcg/kg of GM-CSF and a EPO+GMCSF group (n = 21) which received a combination of the above. Seven animals of each group were killed on the 1st, 3rd and 7th postoperative day and their remnant liver was removed to evaluate liver regeneration by immunochemistry for PCNA and Ki 67.ResultsOur data suggest that EPO and GM-CSF increases liver regeneration following major hepatectomy when administered perioperatively. EPO has a more significant effect than GM-CSF (p < 0.01). When administering both, the effect of EPO seems to fade as EPO and GM-CSF treated rats have decreased regeneration compared to EPO administration alone (p < 0.01).ConclusionEPO, GM-CSF and their combination enhance liver regeneration after hepatectomy in rats when administered perioperatively. However their combination has a weaker effect on liver regeneration compared to EPO alone. Further investigation is needed to assess the exact mechanisms that mediate this finding.

【 授权许可】

CC BY   
© Vassiliou et al; licensee BioMed Central Ltd. 2010