Liver regeneration: immunohistochemichal study of intrinsic hepatic innervation after partial hepatectomy in rats

BMC Gastroenterology

Research Article

Petros Karakitsos¹  Despina Karandrea²  Ioannis Vlachos³  Spyridon Skaltsas³  Alkistis Pantopoulou³  Despina N Perrea³  Marina Palaiologou⁴  Dina G Tiniakos⁵  John Koskinas⁶  Nikolaos Nikiteas⁷  Apostolos N Kandilis⁷ 

[1] Department of Cytopathology, University General Hospital "ATTIKON", School of Medicine, National and Kapodistrian University of Athens, Athens, Greece;Department of Pathology, Aretaieion Hospital, Athens, Greece;Laboratory for Experimental Surgery and Surgical Research "N. S. Christeas", Medical School, University of Athens, Athens, Greece;Laboratory of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece;Laboratory of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece;Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, William Leech Bldg, 4th Floor, Room M4.143, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK;Second Department of Medicine, Medical School, University of Athens, "Hippokration" Hospital, Athens, Greece;Second Propedeutic Department of Surgery, Medical School, University of Athens, General Hospital "Laiko", Athens, Greece;

关键词: Nerve Fiber;    Liver Regeneration;    Orthotopic Liver Transplantation;    Partial Hepatectomy;    Portal Tract;   

DOI  :  10.1186/s12876-014-0202-1

 received in 2014-08-20, accepted in 2014-11-11,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundWe examined the intrinsic hepatic innervation after partial hepatectomy (PH) in rats and the presence and pattern of neural sprouting in regenerating liver.MethodsMale Wistar rats (age 9-13 weeks-w, weight 204-356 g), were submitted to two-thirds PH. Rats were sacrificed at postoperative days (d) 1, 3, 5, 7, at 2 and 4 w, and at 3 and 6 months (m) (6-7 animals/group, control group n = 4). Immunohistochemistry for the pan-neural marker protein gene product 9.5 (PGP9.5) and growth-associated protein 43 (GAP-43), a marker of regenerating nerve axons, was performed on tissue sections from the R1 lobe of the regenerating liver. Portal tracts (PTs) with immunoreactive fibers were counted in each section and computer-assisted morphometric analysis (Image Pro Plus) was used to measure nerve fiber density (number of immuno-positive nerve fibers/mm2 (40x)).ResultsImmunoreactivity for PGP9.5 was positive in all groups. The number of PGP9.5 (+) nerve fibers decreased from 0.32 +/- 0.12 (control group) to 0.18 +/- 0.09 (1d post-PH group), and gradually increased reaching pre-PH levels at 6 m (0.3 +/- 0.01). In contrast, immunoreactivity for GAP-43 was observed at 5d post-PH, and GAP-43 (+) PTs percentage increased thereafter with a peak at 3 m post-PH. GAP-43 (+) nerve fiber density increased gradually from 5d (0.05 +/- 0.06) with a peak at 3 m post-PH (0.21 +/- 0.027). At 6 m post-PH, immunoreactivity for GAP-43 was not detectable.ConclusionsFollowing PH in rats: 1) nerve fiber density in portal tracts decreases temporarily, and 2) neural sprouting in the regenerating liver lobes starts at 5d, reaches peak levels at 3 m and disappears at 6 m post-PH, indicating that the increase in hepatic mass after PH provides an adequate stimulus for the sprouting process.

【 授权许可】

Unknown   
© Kandilis et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.