期刊论文详细信息
BMC Veterinary Research
Diversity of Escherichia coli strains involved in vertebral osteomyelitis and arthritis in broilers in Brazil
Research Article
Roselene Ecco1  Juliana Fortes Vilarinho Braga2  Nathalie Katy Chanteloup3  Angélina Trotereau3  Catherine Schouler3  Sylvie Baucheron3  Rodrigo Guabiraba3 
[1] Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinárias, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Campus Pampulha, 30161-970, Minas Gerais, Brazil;Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinárias, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Campus Pampulha, 30161-970, Minas Gerais, Brazil;ISP, INRA, Université François Rabelais de Tours, 37380, Nouzilly, France;ISP, INRA, Université François Rabelais de Tours, 37380, Nouzilly, France;
关键词: Broilers;    Bacterial infections;    APEC;    Virulence genes;    Pathology;    E. coli;   
DOI  :  10.1186/s12917-016-0762-0
 received in 2016-02-11, accepted in 2016-06-23,  发布年份 2016
来源: Springer
PDF
【 摘 要 】

BackgroundLocomotor disorders and infections by Escherichia coli represent major concerns to the poultry industry worldwide. Avian pathogenic E. coli (APEC) is associated with extraintestinal infections leading to respiratory or systemic disease known as colibacillosis. The most common lesions seen in cases of colibacillosis are perihepatitis, airsacculitis, pericarditis, peritonitis/salpingitis and arthritis. These diseases are responsible for significant economic losses in the poultry industry worldwide. E. coli has been recently isolated from vertebral osteomyelitis cases in Brazil and there are no data on molecular and phenotypic characteristics of E. coli strains isolated from lesions in the locomotor system of broilers. This raised the question whether specific E. coli strains could be responsible for bone lesions in broilers. The aim of this study was to assess these characteristics of E. coli strains isolated from broilers presenting vertebral osteomyelitis and arthritis in Brazil.ResultsFifteen E. coli strains from bone lesions were submitted to APEC diagnosis and setting of ECOR phylogenic group, O serogroup, flagella type, virulence genes content, genetic patterns by Pulsed Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). In addition, bacterial isolates were further characterized through a lethality test, serum resistance test and antibiotic resistance profile. E. coli strains harbored different genetic pattern as assessed by PFGE, regardless of flock origin and lesion site. The strains belonged to seven sequence types (STs) previously described (ST117, ST101, ST131, ST 371 and ST3107) or newly described in this study (ST5766 and ST5856). ECOR group D (66.7 %) was the most frequently detected. The strains belonged to diverse serogroups (O88, O25, O12, and O45), some of worldwide importance. The antibiotic resistance profile confirmed strains’ diversity and revealed a high proportion of multidrug-resistant strains (73 %), mainly to quinolones and beta-lactams, including third generation cephalosporin. The percentage of resistance to tetracycline was moderate (33 %) but always associated with multidrug resistance.ConclusionsOur results demonstrated that vertebral osteomyelitis and arthritis in broilers can be associated with highly diverse E. coli based on molecular and phenotypic characteristics. There was no specific virulence patterns of the E. coli strains associated with vertebral osteomyelitis or arthritis. Also, E. coli strains were frequently multidrug resistant and belonged to STs commonly shared by APEC and human ExPEC strains.

【 授权许可】

CC BY   
© The Author(s). 2016

【 预 览 】
附件列表
Files Size Format View
RO202311108211219ZK.pdf 1412KB PDF download
【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  • [30]
  • [31]
  • [32]
  • [33]
  • [34]
  • [35]
  • [36]
  • [37]
  • [38]
  • [39]
  • [40]
  • [41]
  • [42]
  • [43]
  • [44]
  • [45]
  文献评价指标  
  下载次数:7次 浏览次数:0次