Defense reactions of bean genotypes to bacterial pathogens in controlled conditions

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Uysal, B.^1 ; Bastas, K.K.^2

Igdir University, Faculty of Agriculture, Department of Plant Protection Igdir, Turkey^1

Selcuk University, Faculty of Agriculture, Department of Plant Protection, Konya

42031, Turkey^2

关键词: Ascorbate peroxidase;    Bacterial infections;    Bacterial pathogens;    Bacterial suspensions;    Controlled conditions;    Pseudomonas syringae pv. phaseolicola;    Resistant cultivars;    Xanthomonas axonopodis pv. phaseoli;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012068/pdf
DOI  :  10.1088/1755-1315/142/1/012068

来源: IOP

【 摘 要 】

This study was focused on the role of antioxidant enzymes and total protein in imparting resistance against common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli (Xap) and halo blight caused by Pseudomonas syringae pv. phaseolicola (Psp) in bean. Activities of Ascorbate peroxidase (APX), Catalase (CAT) and total protein were studied in resistant and susceptible bean genotypes. Five-day-old seedlings were inoculated with a bacterial suspension (108CFU ml-1) and harvested at different time intervals (0, 12, 24 and 36 up to 72 h) under controlled growing conditions and assayed for antioxidant enzymes and total protein. Temporal increase of CAT, APX enzymes activities showed maximum activity at 12 h after both pathogens inoculation (hpi) in resistant cultivar, whereas in susceptible it increased at 72 h after both pathogens inoculation for CAT and 12, 24 h for APX enzymes. Maximum total protein activities were observed at 12 h and 24 h respectively after Xap, Psp inoculation (hpi) in resistant and maximum activities were observed at 24 h and 72 h respectively after Xap, Psp inoculation (hpi) in susceptible. Increase of antioxidant enzyme and total protein activities might be an important component in the defense strategy of resistance and susceptible bean genotypes against the bacterial infection. These findings suggest that disease protection is proportional to the amount of enhanced CAT, APX enzyme and total protein activity.