The defense response in Arabidopsis thaliana against Fusarium sporotrichioides

Proteome Science

Research

Takumi Nishiuchi¹  Tomoya Asano²  Makoto Kimura³ 

[1] Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, 920-0934, Kanazawa, Japan;Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, 920-1192, Kanazawa, Japan;Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 13-1 Takaramachi, 920-0934, Kanazawa, Japan;Equipment Support Promotion office, Advanced Science Research Center, Kanazawa University, 920-1192, Kanazawa, Japan;Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN, 2–1 Hirosawa, 351-0198, Wako, Saitama, Japan;

关键词: Arabidopsis thaliana;    Defense response;    Fusarium sporotrichioides;    T-2 toxin;    MAP kinase;    GSTs;    Superoxide dismutase;    Ascorbate peroxidase;   

DOI  :  10.1186/1477-5956-10-61

 received in 2012-07-29, accepted in 2012-10-05,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCertain graminaceous plants such as Zea mays and Triticum aestivum serve as hosts for Fusarium sporotrichioides; however, molecular interactions between the host plants and F. sporotrichioides remain unknown. It is also not known whether any interaction between Arabidopsis thaliana and F. sporotrichioides can occur. To understand these interactions, we performed proteomic analysis.ResultsArabidopsis leaves and flowers were inoculated with F. sporotrichioides. Accumulation of PLANT DEFENSIN1.2 (PDF1.2) and PATHOGENESIS RELATED1 (PR1) mRNA in Arabidopsis were increased by inoculation of F. sporotrichioides. Furthermore, mitogen-activated protein kinase 3 (MPK3) and mitogen-activated protein kinase 6 (MPK6), which represent MAP kinases in Arabidopsis, were activated by inoculation of F. sporotrichioides. Proteomic analysis revealed that some defense-related proteins were upregulated, while the expression of photosynthesis- and metabolism-related proteins was down regulated, by inoculation with F. sporotrichioides. We carried out the proteomic analysis about upregulated proteins by inoculation with Fusarium graminearum. The glutathione S-transferases (GSTs), such as GSTF4 and GSTF7 were upregulated, by inoculation with F. graminearum-infected Arabidopsis leaves. On the other hand, GSTF3 and GSTF9 were uniquely upregulated, by inoculation with F. sporotrichioides.ConclusionsThese results indicate that Arabidopsis is a host plant for F. sporotrichioides. We revealed that defense response of Arabidopsis is initiated by infection with F. sporotrichioides.

【 授权许可】

Unknown   
© Asano et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.