The defense response in Arabidopsis thaliana against Fusarium sporotrichioides

Proteome Science

Takumi Nishiuchi¹  Makoto Kimura²  Tomoya Asano³ 

[1] Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, 920-1192, Japan;Plant & Microbial Metabolic Engineering Research Unit, Discovery Research Institute (DRI), RIKEN, 2–1 Hirosawa, Wako, Saitama, 351-0198, Japan;Equipment Support Promotion office, Advanced Science Research Center, Kanazawa University, Kanazawa, 920-1192, Japan

关键词: Ascorbate peroxidase;    Superoxide dismutase;    GSTs;    MAP kinase;    T-2 toxin;    Fusarium sporotrichioides;    Defense response;    Arabidopsis thaliana;   

Others  :  817161
DOI  :  10.1186/1477-5956-10-61

 received in 2012-07-29, accepted in 2012-10-05,  发布年份 2012

【 摘 要 】

Background

Certain graminaceous plants such as Zea mays and Triticum aestivum serve as hosts for Fusarium sporotrichioides; however, molecular interactions between the host plants and F. sporotrichioides remain unknown. It is also not known whether any interaction between Arabidopsis thaliana and F. sporotrichioides can occur. To understand these interactions, we performed proteomic analysis.

Results

Arabidopsis leaves and flowers were inoculated with F. sporotrichioides. Accumulation of PLANT DEFENSIN1.2 (PDF1.2) and PATHOGENESIS RELATED1 (PR1) mRNA in Arabidopsis were increased by inoculation of F. sporotrichioides. Furthermore, mitogen-activated protein kinase 3 (MPK3) and mitogen-activated protein kinase 6 (MPK6), which represent MAP kinases in Arabidopsis, were activated by inoculation of F. sporotrichioides. Proteomic analysis revealed that some defense-related proteins were upregulated, while the expression of photosynthesis- and metabolism-related proteins was down regulated, by inoculation with F. sporotrichioides. We carried out the proteomic analysis about upregulated proteins by inoculation with Fusarium graminearum. The glutathione S-transferases (GSTs), such as GSTF4 and GSTF7 were upregulated, by inoculation with F. graminearum-infected Arabidopsis leaves. On the other hand, GSTF3 and GSTF9 were uniquely upregulated, by inoculation with F. sporotrichioides.

Conclusions

These results indicate that Arabidopsis is a host plant for F. sporotrichioides. We revealed that defense response of Arabidopsis is initiated by infection with F. sporotrichioides.

【 授权许可】

   
2012 Asano et al.; licensee BioMed Central Ltd.

【 图 表 】

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