In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications

Journal of Nanobiotechnology

Research

Hyun Uk Lee¹  Jouhahn Lee¹  So Young Park¹  Ji-Ho Park²  Moon Il Kim³  Young-Chul Lee³  Yun Suk Huh⁴  Kyoung Suk Kang⁵  Hyun Gyu Park⁵  Sung-Jin Chang⁶  Mino Yang⁷ 

[1] Advanced Nano-Surface Research Group, Korea Basic Science Institute (KBSI), 305-333, Daejeon, Republic of Korea;Department of Bio and Brain Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, 305-701, Daejeon, Republic of Korea;Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, 461-701, Seongnam-si, Gyeonggi-do, Republic of Korea;Department of Biological Engineering, College of Engineering, Inha University, 402-751, Incheon, Republic of Korea;Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, 305-701, Daejeon, Republic of Korea;Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, 156-756, Seoul, Republic of Korea;Division of Analytical Research, Korea Basic Science Institute (KBSI), 200-701, Gangneung, Republic of Korea;

关键词: In-vitro cytotoxicity;    Fe-aminoclay (FeAC);    Carbon nanodots (CD);    Cytotoxicity;    Conjugation;    Bio-imaging;   

DOI  :  10.1186/s12951-015-0151-z

 received in 2015-09-10, accepted in 2015-11-18,  发布年份 2015

来源: Springer

【 摘 要 】

We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role in bio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4′,6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging.Graphical abstractCD conjugation with organo-building blocks of delaminated FeAC NPs

【 授权许可】

CC BY   
© Kang et al. 2015