In-vitro cytotoxicity assessment of carbon-nanodot-conjugated Fe-aminoclay (CD-FeAC) and its bio-imaging applications

Journal of Nanobiotechnology

Hyun Gyu Park²  Young-Chul Lee⁵  Mino Yang⁶  Jouhahn Lee¹  Yun Suk Huh⁷  Ji-Ho Park³  Sung-Jin Chang⁴  So Young Park¹  Moon Il Kim⁵  Hyun Uk Lee¹  Kyoung Suk Kang² 

[1] Advanced Nano-Surface Research Group, Korea Basic Science Institute (KBSI), Daejeon 305-333, Republic of Korea;Department of Chemical and Biomolecular Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea;Department of Bio and Brain Engineering (BK21+ Program), KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea;Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Republic of Korea;Department of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si 461-701, Gyeonggi-do, Republic of Korea;Division of Analytical Research, Korea Basic Science Institute (KBSI), Gangneung 200-701, Republic of Korea;Department of Biological Engineering, College of Engineering, Inha University, Incheon 402-751, Republic of Korea

关键词: Bio-imaging;    Conjugation;    Cytotoxicity;    Carbon nanodots (CD);    Fe-aminoclay (FeAC);    In-vitro cytotoxicity;   

Others  :  1234082
DOI  :  10.1186/s12951-015-0151-z

 received in 2015-09-10, accepted in 2015-11-18,  发布年份 2015

【 摘 要 】

We have investigated the cytotoxic assay of Fe-aminoclay (FeAC) nanoparticles (NPs) and simultaneous imaging in HeLa cells by photoluminescent carbon nanodots (CD) conjugation. Non-cytotoxic, photostable, and CD NPs are conjugated with cationic FeAC NPs where CD NPs play a role inbio-imaging and FeAC NPs act as a substrate for CD conjugation and help to uptake of NPs into cancer cells due to positively charged surface of FeAC NPs in physiological media. As increase of CD-FeAC NPs loading in HeLa cell in vitro, it showed slight cytotoxicity at 1000 μg/mL but no cytotoxicity for normal cells up to concentration of 1000 μg/mL confirmed by two 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red (NR) assays, with further observations by 4′,6-diamidino-2-phenylindole (DAPI) stained confocal microscopy images, possessing that CD-FeAC NPs can be used as potential drug delivery platforms in cancer cells with simultaneous imaging.

【 授权许可】

   
2015 Kang et al.

【 图 表 】

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