Mammary tumour development is dose-dependently inhibited by n-3 polyunsaturated fatty acids in the MMTV-neu(ndl)-YD5 transgenic mouse model

Lipids in Health and Disease

Research

Kate Perez¹  David WL Ma¹  Michael A Leslie¹  Salma A Abdelmagid¹  William J Muller² 

[1] Department of Human Health and Nutritional Sciences, University of Guelph, Animal Science/Nutrition Building, Room 342, 491 Gordon Street, N1G 2W1, Guelph, ON, Canada;Molecular Oncology Labs, McGill University, Royal Victoria Hospital, Montreal, QC, Canada;

关键词: n-3 PUFA;    Eicosapentaenoic acid;    Docosahexaenoic acid;    Mammary gland;    MMTV-neu mice;    Breast cancer;    Tumour;    Tumour multiplicity;    Tumour volume;    Phospholipids;   

DOI  :  10.1186/1476-511X-13-96

 received in 2014-05-01, accepted in 2014-05-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundBreast cancer is attributable to modifiable risk factors including the intake of dietary n-3 polyunsaturated fatty acids (PUFA). A key piece of evidence, yet to be addressed, that would demonstrate a causal relationship between n-3 PUFA and breast cancer, is a dose-dependent effect of n-3 PUFA on tumour outcomes. Thus, the objective of the present study was to determine whether n-3 PUFA reduces mammary gland tumor outcomes in a dose-dependent manner in female MMTV-neu(ndl)-YD5 transgenic mice, an aggressive model of human breast cancer.MethodsHarems were provided one of three experimental diets comprised of 0, 3 or 9% (w/w) menhaden fish oil containing n-3 PUFA. Female offspring were weaned onto the same parental diet and maintained on their respective diet for 20 weeks. Tumour onset, size and multiplicity were measured throughout the study. Fatty acid composition of mammary gland and tumours were determined by gas–liquid chromatography.ResultsTumour size was significantly (p < 0.05) reduced in a dose-dependent manner. n-3 PUFA were also incorporated in a dose-dependent manner; differential incorporation was observed for eicosapentaenoic and docosapentaenoic acids into mammary gland tissue, while docosahexaenoic acid was preferentially incorporated into tumours.ConclusionOverall, the present study provides fundamental knowledge about the dose-dependent effect of n-3 PUFA on tumour outcomes in a pre-clinical model and also sheds light on the differential role of individual n-3 PUFA on tumour outcomes.

【 授权许可】

Unknown   
© Leslie et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.