Generation of aroE overexpression mutant of Bacillus megaterium for the production of shikimic acid

Microbial Cell Factories

Research

Saptarshi Ghosh¹  Uttam Chand Banerjee¹ 

[1] Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S. A. S., 160062, Nagar, Punjab, India;

关键词: Shikimic acid;    Bacillus megaterium;    Shikimate dehydrogenase;    Fermentation;   

DOI  :  10.1186/s12934-015-0251-3

 received in 2014-12-09, accepted in 2015-05-06,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundShikimic acid, the sole chemical building block for the antiviral drug oseltamivir (Tamiflu®), is one of the potent pharmaceutical intermediates with three chiral centers. Here we report a metabolically engineered recombinant Bacillus megaterium strain with aroE (shikimate dehydrogenase) overexpression for the production of shikimic acid.ResultsIn a 7 L bioreactor, 4.2 g/L shikimic acid was obtained using the recombinant strain over 0.53 g/L with the wild type. The enhancement of total shikimate dehydrogenase activity was 2.13-fold higher than the wild type. Maximum yield of shikimic acid (12.54 g/L) was obtained with fructose as carbon source. It was isolated from the fermentation broth using amberlite IRA-400 resin and 89 % purity of the product was achieved.ConclusionThis will add up a new organism in the armory for the fermentation based production which is better over plant based extraction and chemical synthesis of shikimic acid.

【 授权许可】

CC BY   
© Ghosh and Banerjee; licensee BioMed Central. 2015