Identification of the bacterium isolate from Mackerel Fish (Rastrelliger sp.) using 16S rRNA Gene

2nd International Conference on Natural Resources and Life Sciences

生态环境科学

Ismail, Y.S.^1 ; Febriani^2 ; Yulvizar, C.^1 ; Ramadhani, R.^1

Department of Biology, Faculty of Mathematics and Natural Science, Syiah Kuala University, Jl. T. Syech Abdurrauf No.3, Darussalam, Banda Aceh

23111, Indonesia^1

Department of Chemistry, Faculty of Mathematics and Natural Science, Syiah Kuala University, Jl. T. Syech Abdurrauf No.3, Darussalam, Banda Aceh

23111, Indonesia^2

关键词: 16S rRNA sequences;    Bacillus megaterium;    Economic growths;    Neighbor joining;    Nucleotide sequences;    Pathogenic microbes;    Phylogenetic trees;    Probiotic bacteria;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/293/1/012043/pdf
DOI  :  10.1088/1755-1315/293/1/012043

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The use of probiotics in aquaculture can improve the quality and quantity of the fishes. One of potential probiotic bacterium from gastrointestine of mackerel fish (Rastrelliger sp.) isolated in this research were LUI-04. This study aimed to determine the species of bacterium isolate using nucleotide sequence homology compared to other microorganisms in GenBank. The 16S rRNA genes of LUI-04 isolate were analyzed. The results of electrophoresis showed the DNA band had a size of 1 500 bp for amplification using Bact-27F and Uni-1492R primers, and about 250 bp to 500 bp with the Com-IF and Com-2R primers. The sequence of nucleotides was determined by Sanger method. The 16S rRNA sequence of the isolate and other bacteria in GenBank were compared with the Basic Alignment Search Tool (BLAST) to know the homology. Then, all of the sequences were aligned by ClustalW, and the phylogenetic tree was reconstructed with Neighbor Joining method using bootstrap analysis of 1 000 replicates. The results showed that LUI-04 had a homology of 99 % and the bootstrap value percentage of 86 % with Bacillus aryabhattai B8W22 and four strains of Bacillus megaterium (strain QM B1551, NBRC15308, strain IAM 13418 and ATCC 14581). Therefore, the LUI-04 isolate belongs to Bacillus megaterium species.