Characteristics of invasive Acinetobacter species isolates recovered in a pediatric academic center

BMC Infectious Diseases

Research Article

Avish L. Jain¹  Robert S. Munson¹  Chandra L. Shrestha¹  Kaivon Assani¹  Mercedees Haga¹  Christian M. Harding²  Benjamin T. Kopp³  Amy Leber⁴ 

[1] Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA;Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA;Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH, USA;Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA;Nationwide Children’s Hospital, Section of Pulmonary Medicine, 700 Children’s Drive, 43205, Columbus, OH, USA;Department of Laboratory Medicine, Nationwide Children’s Hospital, Columbus, OH, USA;

关键词: Antibiotic resistance;    Bacteremia;    Pediatric;    Acinetobacter;   

DOI  :  10.1186/s12879-016-1678-9

 received in 2015-10-21, accepted in 2016-06-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAcinetobacter species are associated with increasing mortality due to emerging drug-resistance. Pediatric Acinetobacter infections are largely undefined in developed countries and clinical laboratory identification methods do not reliably differentiate between members of the Acinetobacter calcoaceticus-baumannii complex, leading to improper identification. Therefore we aimed to determine risk factors for invasive Acinetobacter infections within an academic, pediatric setting as well as defining microbiologic characteristics of predominant strains.MethodsTwenty-four invasive Acinetobacter isolates were collected from 2009–2013. Comparative sequence analysis of the rpoB gene was performed coupled with phenotypic characterization of antibiotic resistance, motility, biofilm production and clinical correlation.ResultsAffected patients had a median age of 3.5 years, and 71 % had a central catheter infection source. rpoB gene sequencing revealed a predominance of A. pittii (45.8 %) and A. baumannii (33.3 %) strains. There was increasing incidence of A. pittii over the study. Two fatalities occurred in the A. pittii group. Seventeen percent of isolates were multi-drug resistant. A pittii and A. baumannii strains were similar in motility, but A pittii strains had significantly more biofilm production (P value = 0.018).ConclusionsA. pittii was the most isolated species highlighting the need for proper species identification. The isolated strains had limited acute mortality in children, but the occurrence of more multi-drug resistant strains in the future is a distinct possibility, justifying continued research and accurate species identification.

【 授权许可】

CC BY   
© The Author(s). 2016