Molecular markers of antifolate resistance in Plasmodium falciparum isolates from Luanda, Angola

Malaria Journal

Research

Philip J Rosenthal¹  Virgílio E do Rosário²  Filomeno Fortes³  Bianca E Gama⁴  Natália K Almeida de Oliveira⁴  Cláudio Tadeu Daniel-Ribeiro⁴  Maria de Fátima Ferreira-da-Cruz⁴  Florbela JI Lutucuta Kosi⁵  Guilhermina AL Pereira-Carvalho⁶ 

[1] Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, USA;Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal;Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal;National Institute of Public Health, Luanda, Angola;Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil;Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal;Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil;Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal;Department of Biology, Universidade Agostinho Neto, Luanda, Angola;Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil;Health Progress and Investigation Network of the Portuguese-Speaking Countries Community (RIDES/CPLP), Centro de Malária e Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisboa, Portugal;National Institute of Public Health, Luanda, Angola;

关键词: Malaria;    Malaria Case;    Falciparum Malaria;    Malaria Control Programme;    Plasmodium Falciparum Malaria;   

DOI  :  10.1186/1475-2875-10-248

 received in 2011-05-09, accepted in 2011-08-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundPlasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) is widespread, this combination remains an important component of malaria control programmes as intermittent preventive therapy (IPT) for pregnant women and children. In Angola, resistance patterns have been poorly characterized, and IPT has been employed for pregnant women since 2006. The aim of this study was to assess the prevalence of key antifolate resistance mediating polymorphisms in the pfdhfr and pfdhps genes in P. falciparum samples from Angola.MethodsPlasmodium falciparum samples collected in Luanda, in 2007, were genotyped by amplification and DNA forward and reverse sequencing of the pfdhfr and pfdhps genes.ResultsThe most prevalent polymorphisms identified were pfdhfr 108N (100%), 51I (93%), 59R (57%) and pfdhps 437G (93%). Resistance-mediating polymorphisms in pfdhps less commonly observed in West Africa were also identified (540E in 10%, 581G in 7% of samples).ConclusionThis study documents an important prevalence of 4 P. falciparum polymorphisms that predicts an antifolate resistance in Luanda. Further, some samples presented additional mutations associated to high-level resistance. These results suggest that the use of SP for IPT may no longer be warranted in Angola.

【 授权许可】

Unknown   
© Gama et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.