Post - prandial rise of microvesicles in peripheral blood of healthy human donors

Lipids in Health and Disease

Research

Rado Janša¹  Mojca Frank²  Keriya Mam³  Peter Veranič⁴  Eva Ogorevc⁵  Apolonija Bedina-Zavec⁶  Vid Šuštar⁷  Veronika Kralj-Iglič⁷  Roman Štukelj⁷ 

[1] Department of Gastroenterology, University Medical Centre Ljubljana, Japljeva ulica 2, Ljubljana, Slovenia;Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova cesta 62, Ljubljana, Slovenia;FEI Company, Europe NanoPort, Achtseweg Noord 5, 5651, Bldg, GG Eindhoven, The Netherlands;Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Lipičeva ulica 2, Ljubljana, Slovenia;Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška cesta 25, Ljubljana, Slovenia;Laboratory of Biosynthesis and Biotransformation, National Institute of Chemistry, Hajdrihova ulica 19, Ljubljana, Slovenia;Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva ulica 2, Ljubljana, Slovenia;Laboratory of Clinical Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva ulica 2, Ljubljana, Slovenia;

关键词: Blood Glucose Concentration;    Blood Cholesterol;    Total Cholesterol Concentration;    Membrane Raft;    Total Blood Cholesterol;   

DOI  :  10.1186/1476-511X-10-47

 received in 2010-11-21, accepted in 2011-03-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMicrovesicles isolated from body fluids are membrane - enclosed fragments of cell interior which carry information on the status of the organism. It is yet unclear how metabolism affects the number and composition of microvesicles in isolates from the peripheral blood.AimTo study the post - prandial effect on microvesicles in isolates from the peripheral blood of 21 healthy donors, in relation to blood cholesterol and blood glucose concentrations.ResultsThe average number of microvesicles in the isolates increased 5 hours post - prandially by 52%; the increase was statistically significant (p = 0.01) with the power P = 0.68, while the average total blood cholesterol concentration, average low density lipoprotein cholesterol concentration (LDL-C) and average high density lipoprotein cholesterol concentration (HDL-C) all remained within 2% of their fasting values. We found an 11% increase in triglycerides (p = 0.12) and a 6% decrease in blood glucose (p < 0.01, P = 0.74). The post - prandial number of microvesicles negatively correlated with the post - fasting total cholesterol concentration (r = - 0.46, p = 0.035) while the difference in the number of microvesicles in the isolates between post - prandial and post - fasting states negatively correlated with the respective difference in blood glucose concentration (r = - 0.39, p = 0.05).ConclusionsIn a population of healthy human subjects the number of microvesicles in isolates from peripheral blood increased in the post - prandial state. The increase in the number of microvesicles was affected by the fasting concentration of cholesterol and correlated with the decrease in blood glucose.

【 授权许可】

Unknown   
© Šuštar et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.