Dissection of jasmonate functions in tomato stamen development by transcriptome and metabolome analyses

BMC Biology

Research Article

Martin Weyhe¹  Ramona Schubert¹  Susanne Dobritzsch¹  Bettina Hause¹  Julian Dindas²  Gerd Hause³  Joachim Kopka⁴ 

[1] Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle, Germany;Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle, Germany;Present address: Department of Botany I, University of Würzburg, Julius-von-Sachs-Platz 2, D97082, Würzburg, Germany;Martin Luther University Halle Wittenberg, Biocenter, Electron Microscopy, Weinbergweg 22, D06120, Halle, Germany;Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, D14476, Potsdam, (OT) Golm, Germany;

关键词: Array hybridization;    Desiccation;    Ethylene;    Flower development;    Jasmonic acid;    Jasmonic acid-insensitive;    Metabolite profiling;    Pollen development;   

DOI  :  10.1186/s12915-015-0135-3

 received in 2015-03-11, accepted in 2015-03-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundJasmonates are well known plant signaling components required for stress responses and development. A prominent feature of jasmonate biosynthesis or signaling mutants is the loss of fertility. In contrast to the male sterile phenotype of Arabidopsis mutants, the tomato mutant jai1-1 exhibits female sterility with additional severe effects on stamen and pollen development. Its senescence phenotype suggests a function of jasmonates in regulation of processes known to be mediated by ethylene. To test the hypothesis that ethylene involved in tomato stamen development is regulated by jasmonates, a temporal profiling of hormone content, transcriptome and metabolome of tomato stamens was performed using wild type and jai1-1.ResultsWild type stamens showed a transient increase of jasmonates that is absent in jai1-1. Comparative transcriptome analyses revealed a diminished expression of genes involved in pollen nutrition at early developmental stages of jai1-1 stamens, but an enhanced expression of ethylene-related genes at late developmental stages. This finding coincides with an early increase of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) in jai1-1 and a premature pollen release from stamens, a phenotype similarly visible in an ethylene overproducing mutant. Application of jasmonates to flowers of transgenic plants affected in jasmonate biosynthesis diminished expression of ethylene-related genes, whereas the double mutant jai1-1 NeverRipe (ethylene insensitive) showed a complementation of jai1-1 phenotype in terms of dehiscence and pollen release.ConclusionsOur data suggest an essential role of jasmonates in the temporal inhibition of ethylene production to prevent premature desiccation of stamens and to ensure proper timing in flower development.

【 授权许可】

Unknown   
© Dobritzsch et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.