A natural frameshift mutation in Campanula EIL2 correlates with ethylene insensitivity in flowers

BMC Plant Biology

Research Article

Renate Müller¹  Line Jensen¹  Josefine Nymark Hegelund¹  Henrik Lütken¹  Andreas Olsen¹ 

[1]Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegård Allé 9-13, 2630, Taastrup, Denmark

关键词: Campanula;    Deletion;    Ethylene;    Ethylene insensitive;    EIL;    EIN;    Flower development;   

DOI  :  10.1186/s12870-016-0786-4

 received in 2016-03-03, accepted in 2016-04-14,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe phytohormone ethylene plays a central role in development and senescence of climacteric flowers. In ornamental plant production, ethylene sensitive plants are usually protected against negative effects of ethylene by application of chemical inhibitors. In Campanula, flowers are sensitive to even minute concentrations of ethylene.ResultsMonitoring flower longevity in three Campanula species revealed C. portenschlagiana (Cp) as ethylene sensitive, C. formanekiana (Cf) with intermediate sensitivity and C. medium (Cm) as ethylene insensitive. We identified key elements in ethylene signal transduction, specifically in Ethylene Response Sensor 2 (ERS2), Constitutive Triple Response 1 (CTR1) and Ethylene Insensitive 3- Like 1 and 2 (EIL1 and EIL2) homologous. Transcripts of ERS2, CTR1 and EIL1 were constitutively expressed in all species both throughout flower development and in response to ethylene. In contrast, EIL2 was found only in Cf and Cm. We identified a natural mutation in Cmeil2 causing a frameshift which resulted in difference in expression levels of EIL2, with more than 100-fold change between Cf and Cm in young flowers.ConclusionsThis study shows that the naturally occurring 7 bp frameshift discovered in Cmeil2, a key gene in the ethylene signaling pathway, correlates with ethylene insensitivity in flowers. We suggest that transfer of the eil2 mutation to other plant species will provide a novel tool to engineer ethylene insensitive flowers.

【 授权许可】

CC BY   
© Jensen et al. 2016